We narrowed to 19,990 results for: Tec;

1. pDONR221_HUWE1_isoform1

   Plasmid

   #190464

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115209

   Purpose

   Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PbuCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB553

   Plasmid

   #74883

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   No Promoter

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWB544

   Plasmid

   #74878

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_722

   Plasmid

   #216077

   Purpose

   CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Destination

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEL035

   Plasmid

   #137903

   Purpose

   STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. UPB-colon (UPB4)

   Plasmid

   #180786

   Purpose

   Unique Projection Barcode (UPB4)-Colon for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM3Dq ( partial )
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB1H1-Zif268

   Plasmid

   #12612

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

   Insert

   Zif268 in pB1H1 (Egr1 Mouse)
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. RBD#4L

   Plasmid

   #193021

   Purpose

   hnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

   Depositor

   Xue Gao

   Article

   Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

   Insert

   Cas13a
   

   Tags

   hnRNP C RRM

   Expression

   Bacterial

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEC3006 (pSpy1C-PZn_ffluc)

    Plasmid

    #218515

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PZn (PczcD)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. YE2-BE4max

    Plasmid

    #138156

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE2)-nSpCas9-UGI-UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115216

    Purpose

    Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin2Cas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCH68 (dAsCas12a-3xKRAB piggyBac)

    Plasmid

    #217334

    Purpose

    piggyBac expression of dAsCas12a-3xKRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    dAsCas12a
    

    Use

    CRISPR

    Tags

    nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-dCpf1-BE

    Plasmid

    #107685

    Purpose

    Expresses dLbCpf1-BE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE
    

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115208

    Purpose

    Expresses PinCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PinCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-2xFYVE_hrs

    Plasmid

    #67676

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    2xFYVEhrs
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-APOBEC1-YTHD422N

    Plasmid

    #194432

    Purpose

    APOBEC1 fused to YTHD422N

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHD422N (APOBEC1 Human)
    

    Use

    CRISPR

    Tags

    APOBEC1-YTHD422N-HA

    Expression

    Mammalian

    Mutation

    D422N mutation in the YTH domain to enhance m6A b…

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. gRNA NIA Null

    Plasmid

    #198728

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pv6_CBX1_2xChromo

    Plasmid

    #179393

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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