We narrowed to 16,201 results for: genome

Showing: 6841 - 6860 of 16201 results

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Lenti-evoA1-BE4max

Plasmid

#174700

Purpose

Base Editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-BE4max

Use

Lentiviral

Promoter

EFS

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAH750

Plasmid

#91212

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCG150_double_phiC31_attB

Plasmid

#169693

Purpose

cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

Depositor

Shih-Peng Chan

Article

Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

Type

Empty backbone

Expression

Worm

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107320

Purpose

Expresses SpCas9 fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to SaCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-UFD1L

Plasmid

#31560

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

ubiquitin fusion degradation 1-like (UFD1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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Cas12j-8-crRNA

Plasmid

#194967

Purpose

The plasmid expresses Cas12j-8-crRNA expression elements.

Depositor

Yongming Wang

Article

Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Cre-6xHis

Plasmid

#62939

Purpose

Expression of Cre-6xHis in bacterial of cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cre recombinase

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pICH47811-pAtAct2::Cas9-nls::tSlATPase

Plasmid

#160221

Purpose

Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtACT2::Cas9-nls::tSlATPase

Use

Synthetic Biology; Moclo toolkit level 1 acceptor…

Expression

Plant

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSCIP-hCD69-TdTomato

Plasmid

#154095

Purpose

Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Inserts

[5HA]-P2A-tdTomato-[3HA]
hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-PGKpuro2ABFP

Plasmid

#122372

Purpose

Expresses puro-2A-BFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

Puro-2A-BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #2

Plasmid

#139658

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-UFM1-dCS

Plasmid

#139869

Purpose

Mammalian expression of HA-tagged human UFM1-cCS

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Mutation

d84-85

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_KLF9

Plasmid

#86286

Purpose

Donor vector for 3' FLAG tag of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KLF9 homology arms (KLF9 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_2

Plasmid

#86347

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_1

Plasmid

#86346

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBRC_double_attB_GFP_donor

Plasmid

#169692

Purpose

cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)

Depositor

John Wang

Article

Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

Type

Empty backbone

Expression

Worm

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pX335-EN475

Plasmid

#86231

Purpose

For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse CTCF STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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Pinducer20-dCas9-SunTag-2A-BFP

Plasmid

#115779

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

dCas9-SunTag-2A-BFP

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHS638_Flex-Cas12a-CBE

Plasmid

#244845

Purpose

Mammalian expression of Flex-Cas12a cytosine base editor

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-dCas12a-CBE

Use

CRISPR

Tags

2xSV40 NLS-APOBEC3A and Nucleoplasmid NLS-UGI-SV4…

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q/D832A

Promoter

CMV

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX335-EN477

Plasmid

#86232

Purpose

For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse CTCF STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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