We narrowed to 9,499 results for: pho;

1. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

   Plasmid

   #127789

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

   Plasmid

   #127792

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in Arg3…

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTH862-CEN-slowmaxRLuc/minCFLuc

   Plasmid

   #115371

   Purpose

   Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase

   Depositor

   Tobias von der Haar

   Inserts

   Firefly Luciferase (codon minimised)
   Gcn4 5'-UTR + Renilla luciferase (codon optimised)
   

   Expression

   Yeast

   Mutation

   All codons have been changed for the most favoura…

   Promoter

   ADH1 and TDH3

   Available Since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Syn-ChromeT-GFP

   Plasmid

   #123316

   Purpose

   AAV-mediated expression of ChromeT-GFP under the Syn promoter.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

   Promoter

   Syn

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T-3-cytXlATL2

   Plasmid

   #124063

   Purpose

   Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXIATL2
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCHR-CD5HA2-P2A-smAID-mClover

   Plasmid

   #110658

   Purpose

   Lentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degron

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CD5HA2-P2A-AID-mClover
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_NCF2-2/2

   Plasmid

   #91488

   Purpose

   Protein expression and purification of human SH3 domain construct NCF2-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NCF2-2/2 (NCF2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_NCF2-1/2

   Plasmid

   #91479

   Purpose

   Protein expression and purification of human SH3 domain construct NCF2-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NCF2-1/2 (NCF2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Twitch-2C pRSETB

   Plasmid

   #104500

   Purpose

   fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-2C
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pPD3cC

    Plasmid

    #86972

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sema3g.z(S)-AP-His

    Plasmid

    #72027

    Purpose

    Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3g.z (Sema3g Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22-HT/purC

    Plasmid

    #73623

    Purpose

    Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    phosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Contains multiple silent mutations (E179, G185, G…

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sema6a.z-AP-His

    Plasmid

    #72038

    Purpose

    Expresses the extracellular region of the Sema6A, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6a.z (Sema6a Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sema6d.x-AP-His

    Plasmid

    #72047

    Purpose

    Expresses the extracellular region of the Sema6D, isoform x protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.x (Sema6d Mouse, Human)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sema6d.6-AP-His

    Plasmid

    #72046

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 6 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.6 (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Sema6d.z-AP-His

    Plasmid

    #72048

    Purpose

    Expresses the extracellular region of the Sema6D, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.z (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sema6d.2-AP-His

    Plasmid

    #72043

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.2 (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sema3d(S)-AP-His

    Plasmid

    #72018

    Purpose

    Expresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3d (Sema3d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry_C1 I3(resist)

    Plasmid

    #61399

    Purpose

    Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutations of bases C183T T192C T189C

    Promoter

    CMV

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5FRT/TO-GFP-I3(resist)

    Plasmid

    #61400

    Purpose

    Expresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    silent mutations of bases C183T T192C T189C

    Promoter

    CMV

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only