We narrowed to 174,943 results for: Mal

1. pLenti_Ef1a:PE2-P2A-GFP

   Plasmid

   #184445

   Purpose

   Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

   Depositor

   Bobby Koeleman

   Article

   Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and Ef1A

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. WN10150

   Plasmid

   #80443

   Purpose

   Expresses dead (908A) AsCpf1

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   nuclease dead (908A) AsCpf1
   

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   nuclease dead (D908A)

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Il12b 400bp pro-Luc

   Plasmid

   #20020

   Depositor

   Stephen Smale

   Article

   Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

   Insert

   Il12b promoter (Il12b Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLOVE-Nmyc

   Plasmid

   #15951

   Depositor

   Miguel Ramalho-Santos

   Article

   Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

   Insert

   v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   The recombination reaction required to clone &quo…

   Available Since

   Nov. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

5. PD-1-miSFIT-4x

   Plasmid

   #124678

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUGW-RUSH-LAMP2A-mNeonGreen

   Plasmid

   #228525

   Purpose

   Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.

   Depositor

   Ginny Farias

   Article

   Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

   Insert

   LAMP2 (Lamp2 Rat)
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP), Streptavidin-…

   Expression

   Mammalian

   Promoter

   Ubc

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

   Plasmid

   #170248

   Purpose

   Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Mutation

   C124S

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-PIF3-mEGFP

   Plasmid

   #100283

   Purpose

   Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome interacting factor 3 (PIF3 Mustard Weed)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N-terminus (1-100 aa) of PIF3 is used

   Promoter

   CAG promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER-21-SPI1

   Plasmid

   #97039

   Purpose

   Expresses SPI1 in mammalian cells

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Spi-1 Proto-Oncogene (SPI1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Tet on

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

10. MO91-dCas9-AID(P182X)

    Plasmid

    #83260

    Purpose

    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

    Depositor

    Xing Chang

    Article

    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

    Insert

    dCas9-AID P182X
    

    Use

    Retroviral

    Tags

    3xFLAG-NLS and FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9; P182X in AID

    Promoter

    MSCV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pSH231-EF1-BLST-dCas9-VPR

    Plasmid

    #115148

    Purpose

    Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    BlastR-P2A-dCas9-VPR
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LV_EF1a_CCNB1-P2A-Hygro_Barcode

    Plasmid

    #170218

    Purpose

    Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CCNB1 (CCNB1 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cre-p2a-tdTomato

    Plasmid

    #176837

    Purpose

    Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Cre-P2A-tdTomato
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcBac1-Pyl-TAG

    Plasmid

    #228784

    Purpose

    Pyl aaRS/tRNA system for mammalian TAG suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    PylRS
    PytR
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

    Plasmid

    #89874

    Purpose

    expresses DRD2-iTango component in mammalian cells for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    Human synapsin

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJZC34

    Plasmid

    #62331

    Purpose

    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2(wt+f6) binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSicoR-Ef1a-mCh

    Plasmid

    #31847

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

    Plasmid

    #174896

    Purpose

    Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CKIIa-stGtACR1-FusionRed

    Plasmid

    #105679

    Purpose

    Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-EF1A-Puro-mCherry-RAB7

    Plasmid

    #221555

    Purpose

    Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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