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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 157,779 results for: ins

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Showing: 6901 - 6920 of 157779 results

  1. pDsRed-attP

    Plasmid
    #51019
    Purpose
    Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Article
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    Use
    High copy, amp resistance
    Promoter
    3xP3 and None
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51019-plasmid-map-sequence-id-222558

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  2. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  8. pMV306DIhsp+LuxG13

    Plasmid
    #49999
    Purpose
    Integrase removed to increase stability of the plasmid in mycobacteria
    Depositor
    Brian Robertson
    Article
    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2
    Insert
    Bacterial luciferase operon + G13 promoter
    Expression
    Bacterial
    Mutation
    It contains a gram-positive enhanced translation …
    Promoter
    G13 from M. marinum G13 promoter driving luxC
    Available Since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    49999-plasmid-map-sequence-id-74814

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  10. pCsy_complex

    Plasmid
    #89232
    Purpose
    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Article
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Inserts
    Csy1
    Csy2
    Csy3
    Csy4
    Tags
    6xHis-TEV protease
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89232-plasmid-map-sequence-id-177172

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  13. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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  14. pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174640
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-GFP-GCN4-ppKinVIb(861-1321)
    Tags
    SSPB(micro)-GFP
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174640-plasmid-map-sequence-id-347051

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  17. pAAV-hSyn-GRAB_NE1m

    Plasmid
    #123308
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE sensor GRAB_NE1m
    Use
    AAV
    Mutation
    contains a glycine-to-threonine mutation at posit…
    Promoter
    hSyn
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123308-plasmid-map-sequence-id-240666

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Showing: 6901 - 6920 of 157779 results