161,398 results

1. pM2s2AsG

   Plasmid

   #137924

   Purpose

   E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   Superfolder GFP
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDrVSP-IRES2-EGFP

   Plasmid

   #80333

   Purpose

   Expresses Danio rerio VSP with separated EGFP in mammalian cell

   Depositor

   Yasushi Okamura

   Article

   Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

   Insert

   Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHB-4

   Plasmid

   #140957

   Purpose

   For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

   Depositor

   Kang Zhou

   Article

   Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

   Insert

   phaCAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-TRE3G-dCas9-EGFP-HaloTag

   Plasmid

   #169451

   Purpose

   expression of dCas9-EGFP-HaloTag

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9-EGFP-HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMThy1.1_hDDX5_FL

   Plasmid

   #88873

   Purpose

   constitutive expression of DDX5 in mammalian cells

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-p18-CIBN

   Plasmid

   #188990

   Purpose

   A lentiviral plasmid encoding lysosome-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Use

   Tags

   p18

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-NLS-GFP (AAV PHP.eB)

   Viral Prep

   #104061-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Promoter

   CAG

   Tags

   GFP

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pHR CD19-FcGamma CAR

   Plasmid

   #113014

   Purpose

   for production of lentivirus encoding the CD19 CAR-Pfcgamma

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P intracellular GFP (CD19 Human, Synthetic, Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

    Plasmid

    #194685

    Purpose

    CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-racRNA-A

    Plasmid

    #200824

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-VSVG

    Plasmid

    #11912

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L, S65T, A206K relative the wild type GFP sequ…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. RCASBP-AP (A) (CC#37)

    Plasmid

    #15160

    Depositor

    Connie Cepko

    Article

    Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

    Insert

    PLAP (ALPP Human)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Neo-Bam p53 wt

    Plasmid

    #16434

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-21a-PE2-His

    Plasmid

    #201973

    Purpose

    Express His-tagged PE2 in bacteria

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    PE2-HIS
    

    Use

    Tags

    His and c-myc NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-gCarvi-T2A

    Plasmid

    #190333

    Purpose

    Fluorescent reporter for cAMP

    Depositor

    Naoto Saitoh

    Article

    Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    gCarvi
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. a1Ha-pcDNA3

    Plasmid

    #45809

    Depositor

    Edward Perez-Reyes

    Article

    Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

    Insert

    a1Ha (CACNA1H Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. TFORF1938

    Plasmid

    #141892

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MEIS1 (MEIS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. human MMP-9 promoter

    Plasmid

    #53434

    Purpose

    promoter activity assay

    Depositor

    Etty Benveniste, Douglas Boyd

    Article

    Ma et al J Immunol. 2001 Nov 1;167(9):5150-9.

    Insert

    MMP-9 promoter (MMP9 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    MMP9

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCDNA3 Flag p38 delta

    Plasmid

    #20785

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    pDCNA3 Flag p38 delta (Mapk13 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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24. CAG-GFP

    Plasmid

    #16664

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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25. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pR-mKate2

    Plasmid

    #228610

    Purpose

    Mammalian expression of mKate2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

    Insert

    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. CD4-MKII-hLOV1-TEVcs(ENLYFQ/M)- Gal4

    Plasmid

    #163026

    Purpose

    FLiCRE TF for HEK293T expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    CD4-MKII-hLOV1-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pK031.TRE-Cre (Supernova)

    Plasmid

    #69136

    Purpose

    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

    Depositor

    Takuji Iwasato

    Article

    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

    Insert

    NLS-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBUE411-GGB

    Plasmid

    #198233

    Purpose

    Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411

    Depositor

    Andrea Gallavotti

    Inserts

    GRF4-GIF1 chimera cassette
    pPLTP::BBM cassette
    

    Use

    CRISPR; Plant binary vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin and maize PLTP

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. lenti-RfxCas13d-Blast-BFP

    Plasmid

    #196726

    Purpose

    Sortable and selectable RfxCas13d.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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