We narrowed to 37,143 results for: Tec;

1. L2-AA012

   Plasmid

   #185751

   Purpose

   Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

   Depositor

   Peter Szoevenyi

   Article

   Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

   Inserts

   HygR2
   eGFP
   

   Tags

   membrane localization tag Lti6b

   Expression

   Plant

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 NFKB1

   Plasmid

   #67126

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pUPD2 AtUBQ10 (GB2478)

   Plasmid

   #160594

   Purpose

   UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtUBQ10
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CTA2-mNG211

   Plasmid

   #141167

   Purpose

   Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   SUMO-CTA2-mNG211
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB3041(gRNA X-3)

   Plasmid

   #73283

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. LbABE8e-dimer

   Plasmid

   #138505

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-dLbCas12a
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET FLIPglu-600uDelta11 Aphrodite

   Plasmid

   #13569

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A Delta11 Aphrodite
   

   Tags

   Aphrodite and ECFP

   Expression

   Bacterial

   Mutation

   Fluorophore-derived linker shortened. F16A mutati…

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G03 DDX5

   Plasmid

   #67111

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRSETa-Amrose v3

   Plasmid

   #79539

   Purpose

   pRSETa (Invitrogen) plasmid expressing Amrose variant 3

   Depositor

   Randolph Caldwell

   Article

   Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

   Insert

   Amrose v3
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIII-Neo-E2Crimson

    Plasmid

    #153425

    Purpose

    Retroviral (MSCV) expression of E2-Crimson fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    E2-Crimson
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK233 (AAVS1 CMV-AtAFB2)

    Plasmid

    #140537

    Purpose

    AAVS1 CMV-AtAFB2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-AtAFB2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB3047(gRNA XII-1)

    Plasmid

    #73289

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 CTTN

    Plasmid

    #67100

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTTN (CTTN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTS2353_Tier3(PB)-YPet_PuroR

    Plasmid

    #169652

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TAL2295

    Plasmid

    #36728

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    APC-TALEN_Right (APC Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pJL1-TTlight-4xDQNAT

    Plasmid

    #128394

    Purpose

    In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin light chain domain
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    Inactivating E234A mutation

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBICI_2_strong

    Plasmid

    #155168

    Purpose

    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-MBP-(CBX1)3-eReader

    Plasmid

    #135090

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered x3
    

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

    Plasmid

    #191177

    Purpose

    Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:GAPDH.s1:EGFP (frameshifted)
    

    Expression

    Mammalian

    Mutation

    EGFP is frameshifted

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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