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We narrowed to 11,947 results for: emb

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Showing: 6961 - 6980 of 11947 results

  1. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

    Plasmid
    #203802
    Purpose
    Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    RAB10 (RAB10 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    T23N
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203802-plasmid-map-sequence-id-401645

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  4. pCMV_SEPT2 NCmut-msfGFP

    Plasmid
    #180320
    Purpose
    mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP
    Depositor
    Manos Mavrakis
    Publication
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SEPTIN2 (SEPTIN2 Human)
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    SEPT2 F20D, V27D
    Promoter
    CMV
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    180320-plasmid-map-sequence-id-358053

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  8. pcry:GFP,-503unc:hACTA1GFP

    Plasmid
    #172529
    Purpose
    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.
    Depositor
    Joachim Berger
    Publication
    Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATCA1 (ACTA1 Human)
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172529-plasmid-map-sequence-id-341033

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  9. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Publication
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

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  10. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

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  11. prNFL-P8R-EGFP

    Plasmid
    #132598
    Purpose
    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation
    Depositor
    Anthony Brown
    Publication
    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nefl (Nefl Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    23C>G; Pro8Arg
    Promoter
    CMV
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132598-plasmid-map-sequence-id-260971

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  12. prNFL-E397K-EGFP

    Plasmid
    #132603
    Purpose
    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation
    Depositor
    Anthony Brown
    Publication
    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nefl (Nefl Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    1189G>A; Glu397Lys
    Promoter
    CMV
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132603-plasmid-map-sequence-id-260029

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  13. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid
    #131138
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Mark Peifer
    Publication
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    Crk (Crk Fly)
    Tags
    3XFLAG and tdTomato
    Expression
    Insect
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131138-plasmid-map-sequence-id-258855

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  16. pUC19-K1LM04

    Plasmid
    #103119
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103119-plasmid-map-sequence-id-201919

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  17. pUC19-E3CY56

    Plasmid
    #103075
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103075-plasmid-map-sequence-id-201714

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Showing: 6961 - 6980 of 11947 results