We narrowed to 59,357 results for: cloning

1. pD(P+T)_T7N

   Plasmid

   #223855

   Purpose

   4G-cloning donor for bacterial promoter and terminator, N-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD(P+T)_T7C

   Plasmid

   #223856

   Purpose

   4G-cloning donor for bacterial promoter and terminator, C-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD(Nt)_His10-TS-3C

   Plasmid

   #223884

   Purpose

   4G-cloning donor for N-terminal His10-TwinStrep-3C tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His10-TwinStrep-3C
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD(Ct)_3C-Venus-His8

   Plasmid

   #223889

   Purpose

   4G-cloning donor for C-terminal 3C-Venus-His8 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3C-Venus-His8
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD(P+T)_CMVC

   Plasmid

   #223862

   Purpose

   4G-cloning donor for mammalian promoter and terminator, C-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   CMV promoter and bGH polyA
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p667-UBC-STK17A-V5-miniTurbo_IDG-K

   Plasmid

   #214466

   Purpose

   Gateway destination clone of STK17A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   STK17A-V5-miniTurbo (STK17A Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp LYS2-MET17

   Plasmid

   #122910

   Purpose

   LYS2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIp LYS2-URA3

   Plasmid

   #122911

   Purpose

   LYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIp TRP1-LEU2

   Plasmid

   #122913

   Purpose

   TRP1-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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10. YIp TRP1-MET17

    Plasmid

    #122914

    Purpose

    TRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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11. YIp LYS2-KanMx

    Plasmid

    #122920

    Purpose

    LYS2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. R777-E185 Hs.PLXNB1

    Plasmid

    #70469

    Purpose

    Gateway ORF clone of human PLXNB1 [NM_001130082.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PLXNB1 (PLXNB1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. R777-E221 Hs.RALGDS

    Plasmid

    #70505

    Purpose

    Gateway ORF clone of human RALGDS [NM_006266.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RALGDS (RALGDS Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-UBC-irfp luc-EF1 Hygro

    Plasmid

    #129438

    Purpose

    express irfp-luc chimera

    Depositor

    Oskar Laur

    Insert

    irfp-luc
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-UBC-dendra 2LUC-EF1 Hygro

    Plasmid

    #129439

    Purpose

    express dendra2-luc chimera

    Depositor

    Oskar Laur

    Insert

    dendra2-luc
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-UBC-DSRED-LUC-EF1 Hygro

    Plasmid

    #129437

    Purpose

    express dsred-luc chimera

    Depositor

    Oskar Laur

    Insert

    dsred-luc
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. R777-E287 Hs.ROS1

    Plasmid

    #70571

    Purpose

    Gateway ORF clone of human ROS1 [NM_002944.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    ROS1 (ROS1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMVlac-dirTopo-P2A-aGFP

    Plasmid

    #61972

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p28BIOHTEV-LIC

    Plasmid

    #167852

    Purpose

    Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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