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pC4-RHE-PAS (FRB-PAS)

Plasmid

#80002

Purpose

miRFP PAS domain fused to FRB

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FRB

Tags

PAS domain of miRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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p1.1

Plasmid

#162737

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2_169

Plasmid

#199101

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_173

Plasmid

#199105

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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p043 mTcf-4 B

Plasmid

#11031

Depositor

Ramesh Shivdasani

Article

Lee et al J Biol Chem. 1999 Jan 15. 274(3):1566-72

Insert

Tcf-4 (Tcf7l2 Mouse)

Use

Xenopus, avian, and zebrafish expression

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pC4EN-F1-mGAF670 (FKBP-mGAF670)

Plasmid

#80003

Purpose

miRFP670 GAF domain fused to FKBP

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FKBP

Tags

GAF domain of miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT13.G

Plasmid

#201001

Purpose

pLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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TC1078-30

Plasmid

#164878

Purpose

30bp(mismatch17) cas13d gRNA for 1405 editing

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

gRNA for TC1405

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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A0A075HNX4

Plasmid

#177695

Purpose

expresses a fatty alcohol oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

Long chain alcohol oxidase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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FCK-QuasAr2-mOrange2 eFRET

Plasmid

#59173

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Article

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-mOrange2 eFRET

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSB19-PapRS#34-OmpA

Plasmid

#231105

Purpose

Vector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)

Depositor

Arne Skerra

Article

Mayrhofer et al Nat Commun. 2024 Dec 18;15(1):10693. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DARPinOFF7

Plasmid

#182396

Purpose

Bacterial expression of DARPinOFF7

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DARPinOFF7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX165-Cas9-HF1

Plasmid

#140565

Purpose

Expresses Cas9-HF1 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

N497A R661A Q695A Q926A

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6d

Plasmid

#215832

Purpose

Express CBE6d (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-eNme2C-2xUGI

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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MXS_CMV

Plasmid

#62417

Purpose

MXS_chaining vector with CMV

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAB143

Plasmid

#101679

Purpose

LAA-tagged mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

LAA-tagged mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CytERM-dTomato

Plasmid

#98834

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

dTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)

Plasmid

#80004

Purpose

miRFP709 GAF domain fused to FKBP and NLS

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FKBP

Tags

GAF domain of miRFP709 and NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pT2/SVNeo-103

Plasmid

#79841

Purpose

Expresses RpBphP1-mCherry-TetR under CMVd1 promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

RpBphP1-mCherry-Intersectin1 DHPH domain

Expression

Mammalian

Promoter

CMVd1

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-gRNA2.1

Plasmid

#167161

Purpose

Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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