We narrowed to 81,743 results for: Mycs

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pWDY1226

Plasmid

#138593

Purpose

Expresses NatL2-R156A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Arginine 156 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1222

Plasmid

#138592

Purpose

Expresses NatL2-K418A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Lysine 418 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pUG-UBC4

Plasmid

#104668

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC4 (UBC4 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-10X-tRNA

Plasmid

#70123

Purpose

Gateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAs

Depositor

Jay Hesselberth

Article

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Insert

10X-tRNA block

Promoter

None – SUP4 pol III promoter provided by insert

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
pAD06 K. lactis telomerase RNA hairpin

Plasmid

#61895

Purpose

Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin (nt 229-273)

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5'ETS 923 to 1179

Plasmid

#23995

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rRNA 5'ETS ntds 923 to 1179

Use

Bacterial cloning - topo

Mutation

None

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5'ETS 1 to 177

Plasmid

#23993

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA 5'ETS ntds 1 to 177

Use

Bacterial cloning - topo

Mutation

None

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-25S rDNA

Plasmid

#23996

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

25S rRNA from +5270 to oligo y

Use

Bacterial cloning - topo

Mutation

none

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pET-sCS4T

Plasmid

#28249

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain Cse4-Scm3-H4 with Thrombin site

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between Cse4 and Scm3

Available Since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pET-sCS4T+N

Plasmid

#28255

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

sCS4T+N

Tags

His

Expression

Bacterial

Mutation

single chain CSE4-SCM3-H4 with Thrombin site, SCM…

Available Since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pET-sC4T

Plasmid

#28251

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain Cse4-H4 with Thrombin site

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between Cse4 and H4

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pGMEH-P38-luxAFB-DAS+4

Plasmid

#27064

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxA-FLAG-luxB-DAS+4

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO SSA1

Plasmid

#19462

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCpSRb-4

Plasmid

#13726

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

Circularly permuted SRβD210K183 with Precission p…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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L284F Cus2p

Plasmid

#73059

Purpose

S. cerevisiae Cus2p gene with L284F Mutation

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 Dec 2.

Insert

CUS2p (CUS2 Budding Yeast)

Tags

6x His tag fused to TEV cleavage site

Expression

Bacterial and Yeast

Mutation

Leucine 284 to Phenylalanine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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D282N Cus2p

Plasmid

#73060

Purpose

S. cerevisiae Cus2p gene with D282N Mutation

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 Dec 2.

Insert

CUS2p (CUS2 Budding Yeast)

Tags

6x His tag fused to TEV cleavage site

Expression

Bacterial and Yeast

Mutation

Asp 282 to Asn

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2

Plasmid

#52961

Purpose

Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

Spiralin

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQAT39

Plasmid

#223411

Purpose

T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN(CMV-copGFP-Puro-SMAR)

Plasmid

#80391

Purpose

expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

Depositor

Magnus Essand

Article

Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

Insert

copGFP-T2A-Puromycine

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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