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We narrowed to 14,065 results for: LEA

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Showing: 7121 - 7140 of 14065 results

  1. XanthoMoClo Xanthobacter Genetic Toolkit

    Plasmid kit
    #1000000259
    Purpose
    Plasmids and parts for modular assembly of single and multi-gene plasmids for any Xanthobacter or Roseixanthobacter strain.
    Depositor
    Michael Springer
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only

  2. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

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  3. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

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  4. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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  6. pMTB-Multibow-mC

    Plasmid
    #60986
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound cerulean
    Depositor
    Sean Megason
    Publication
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    membrane tag
    Promoter
    Bactin2
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pHL 1477

    Plasmid
    #69787
    Purpose
    KanR with FRT sites + ptet::OmpC::T7RNAP::T1 terminator + ColE
    Depositor
    Han Lim
    Publication
    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw
    Insert
    T7RNAP
    Use
    Synthetic Biology
    Tags
    OmpC leader
    Expression
    Bacterial
    Promoter
    pTet
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pExp-T7tag

    Plasmid
    #129246
    Purpose
    Protein expression in E.coli
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    His and Strep
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129246-plasmid-map-sequence-id-252354

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Showing: 7121 - 7140 of 14065 results