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pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT3.0mut

Plasmid

#208718

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SLC38A9.1

Plasmid

#71869

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Tags

HA

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB_g5-HT3.0mut

Plasmid

#208712

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLacO-pEF1α-RFP

Plasmid

#179507

Purpose

Expresses monomeric RFP in mammalian cells under a EF1α promoter

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

monomeric RFP

Expression

Mammalian

Promoter

EF1α

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-NLuc

Plasmid

#135953

Purpose

Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

2xSV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

Plasmid

#218767

Purpose

encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-pAAFRS-9

Expression

Bacterial

Mutation

Y37G, L71V, D182C, F183Y, L186C, D265R

Promoter

tacI

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-mCherry

Plasmid

#139606

Purpose

Expresses a VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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SGLT2(GFP)-MAP17(nb)

Plasmid

#216211

Purpose

for expression of human GFP-tagged SGLT2-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT2 GFP fusion and MAP17 nanobody fusion (PDZK1IP1, SLC5A2 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc)

Plasmid

#160368

Purpose

Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURO

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-myc

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

Plasmid

#194683

Purpose

CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/hArf4(T31N)-HA

Plasmid

#79405

Purpose

Expresses C-terminally HA-tagged Arf4(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(Q71L)-HA

Plasmid

#79404

Purpose

Expresses C-terminally HA-tagged Arf4(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

HA

Expression

Mammalian

Mutation

Gln 71 Leu

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(WT)-HA

Plasmid

#79403

Purpose

Expresses C-terminally HA-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

HA

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

Plasmid

#164612

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Expression

Mammalian

Mutation

S383T, F177A

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

Plasmid

#115267

Purpose

Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

LbuCas13a

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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PMXS-SLC1A3

Plasmid

#72873

Purpose

The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

SLC1A3 (SLC1A3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLHCX-FLAG-mPKM2

Plasmid

#44239

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M2 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only