We narrowed to 64,643 results for: cell;

1. pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

   Plasmid

   #197044

   Purpose

   In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

   Depositor

   Liqun Luo

   Article

   Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

   Inserts

   pCAG-FSF-LSL
   SP-HA-HRPtm
   WPRE-bGHpolyA
   

   Tags

   HA, myc

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF6-Acly

   Plasmid

   #70765

   Purpose

   Expresses mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF6-Acly(S455D)

   Plasmid

   #70768

   Purpose

   Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   S455D

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF6-Acly(S455A)

   Plasmid

   #70767

   Purpose

   Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   S455A

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV40-EF1a-hBCL10-SO

   Plasmid

   #75256

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Promoter

   pMSCV-LTRs, EF1a

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-601_NY-ESO-1_TCR_GFP

   Plasmid

   #207482

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   GFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Mutation

   Potential silent point mutation in GFP sequence

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-TRE-MA4-GFP

   Plasmid

   #114380

   Purpose

   Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

   Depositor

   Yuet Wai Kan

   Article

   Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   MLL-AF4 fusion gene (AFF1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-602_NY-ESO-1_TCR_RFP

   Plasmid

   #207483

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv2_mSTING_gRNA_1

   Plasmid

   #196625

   Purpose

   Knock-out STING in murine cells, gRNA 1.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING gRNA 1 (Sting1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pINDUCER-21-RUNX1

    Plasmid

    #97043

    Purpose

    Expresses RUNX1 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1 (RUNX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pLentiCRISPRv2_mSTING_gRNA_2

    Plasmid

    #196626

    Purpose

    Knock-out STING in murine cells, gRNA 2.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING gRNA 2 (Sting1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-hACE2-hygro

    Plasmid

    #161758

    Purpose

    Expresses human ACE2 in mammalian cells

    Depositor

    Neville Sanjana

    Article

    Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    P2A

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Phage-PGK-Flag-HA-FKBP-T(WT)

    Plasmid

    #181734

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Article

    Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Fucci(CA) hCdt1-iRFP hGeminin-iRFP

    Plasmid

    #190182

    Purpose

    Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    iRFP
    hGeminin(1/110)
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti.pFOXA2.GFP

    Plasmid

    #59404

    Purpose

    expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    400 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pFOXA2

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXS-IRES-Blast mAtg7

    Plasmid

    #173170

    Purpose

    Retroviral vector to express sgRNA resistant mouse Atg7

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-SIN-BX SpyCatcher003-(short)mCD80

    Plasmid

    #210567

    Purpose

    Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP71-hPDL-1

    Plasmid

    #118631

    Purpose

    human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

    Depositor

    Sébastien Walchli

    Article

    Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

    Insert

    hPDL-1 (CD274 Human)
    

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc-mCh-Tub

    Plasmid

    #24288

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Alpha-Tubulin (alphaTub84B Fly)
    

    Tags

    mCherry

    Expression

    Insect

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

20. Lenti.pFOXA2.5'.GFP

    Plasmid

    #59407

    Purpose

    expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    FOXA2

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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