-
-
pcDNA-Dest53-SHP-1 WT
Plasmid#21791DepositorInsertSHP-1 (PTPN6 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-deSpCas9
Plasmid#92114PurposeExpression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)DepositorInsertdead/inactive eSpCas9 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, H840A, K848A, K1003A, R1060APromoterCbhAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
854-Desmocollin 1a-myc
Plasmid#32231DepositorInsertDesmocollin 1a (DSC1 Human)
UseTagsMycExpressionMammalianMutationPromoterhuman beta-actinAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:tdTomato
Plasmid#135214PurposeRed tagged macrophagesDepositorInsertmfap4 promoter and tdTomato
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN0604 (pDEST, HygR)
Plasmid#210483PurposepDEST vector with HygR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0603 (pDEST, NatR)
Plasmid#210482PurposepDEST vector with NatR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti Dest PuroR R1-R2
Plasmid#84575PurposeGateway destination vector expressing Puromycin resistance under a PGK promoterDepositorTypeEmpty backboneUseLentiviral; GatewayTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-FASCIN
Plasmid#31207DepositorInsertFASCIN (FSCN1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCK081 desmaMCS:minpromCerulean,exorh:mCherry
Plasmid#200014PurposeExpression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherryDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-Y1699C
Plasmid#25048DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationTyrosine 1699 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab33bb in pCSDEST2
Plasmid#140882PurposeExpression of zebrafish GFP-tagged Rab33bb in mammalian cells.DepositorInsertRab33bb
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51-GOLPH3
Plasmid#21690DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseInvitrogen gateway destination vectorTags6xHIS and V5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-cDEST-APEX2
Plasmid#214993PurposeGateway compatible empty vector (lentiviral/mammalian expression) with a C-terminal APEX2 tagDepositorTypeEmpty backboneUseLentiviralTagsAPEX2-FlagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST53-mTrific
Plasmid#51031Purposemammalian expression of mouse Trific fused to GFPDepositorInsertTrific (Trim46 Mouse)
UseTagsCycle3 GFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-FLAG
Plasmid#119754PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDest Lenti CA-GFP
Plasmid#197459PurposeBicistronic expression vector expressing CA-GFP under the CMV promotor separated by an internal ribosome entry site (IRES) mediated expression of dTomato reporter protein.DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationS65TPromoterCMV, IRESAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SARA-GFP
Plasmid#67410PurposeMammalian expression of hSara with C-term GFP tagDepositorInsertSARA (SAR1B Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF6-GFP
Plasmid#67394PurposeMammalian expression of hArf6 with C-term GFP tagDepositorInsertARF6 (ARF6 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest eGFP CUGBP1
Plasmid#61276PurposeMammalian expression of EGFP-tagged human CUGBP1DepositorInsertCUG-BP (CELF1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-R1441C
Plasmid#25081DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationArginine 1441 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
pDEST-EGFP-TARG1
Plasmid#172586PurposeFor transient expression of N-terminal GFP-tagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-P110alpha
Plasmid#40230DepositorInsertP110 alpha (PIK3CA Bovine)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-alpha-catenin
Plasmid#20139DepositorInsertpDEST27-alpha catenin
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
1456_pDEST_miniTol2_R4-R2_Cryst-eGFP
Plasmid#171795PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hyper7 in pT3TS-Dest
Plasmid#194296PurposeIn vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRLDepositorInsertHyper7
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBM-mut
Plasmid#187150Purposemammalian cell expression of FLAG-HUWE1 L2976A/P2977ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationL2976A/P2977APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UIM-mut
Plasmid#187148Purposemammalian cell expression of FLAG-HUWE1 A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationA1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA-mut
Plasmid#187146Purposemammalian cell expression of FLAG-HUWE1 M1328A/F1330ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationM1328A/F1330APromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
371 pTol2-fli1ep:MTDest
Plasmid#73490PurposeTol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signalDepositorInsertfli1ep:6xMyc-attR1-attR2
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
IQ013: pMVP/Ad-DEST
Plasmid#121843PurposepMVP destination vector, empty adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
726-Desmoglein2-myc
Plasmid#32232DepositorInsertDesmoglein2 (DSG2 Human)
UseTagsMycExpressionMammalianMutationPromoterhuman beta-actinAvailable sinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTripz NEO DEST
Plasmid#209072PurposeModified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interestDepositorTypeEmpty backboneUseLentiviral; Gateway destination vector to be used…Tags3X FLAGExpressionMutationPromoterCMVAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorUseTagsGSTExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
KX002: pMVP/PB-DEST
Plasmid#121869PurposepMVP destination vector, empty PiggyBac transposon vector backboneDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Myc
Plasmid#119752PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.DepositorTypeEmpty backboneUseTagsGST and MycExpressionBacterialMutationPromotertac promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-KD
Plasmid#140313PurposeExpresses Flag-tagged human Src-KD in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationContains only the KD regionPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL1-GFP
Plasmid#67395PurposeMammalian expression of hArl1 with C-term GFP tagDepositorInsertARL1 (ARL1 Human)
UseTagsGFPExpressionMammalianMutationbp 231 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only