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Plasmid#74287PurposepAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent mannerDepositorInsertPBG (chimeric rabies Glycoprotein)
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
MERS-CoV spike all7KO mutant
Plasmid#231924PurposeFor expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225, N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutationDepositorInsertspike all7KO (S MERS-CoV)
UseTagsExpressionMammalianMutationN1176Q, N1213Q, N1225Q, N1241Q, N1256Q, N1277Q, N…PromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn PGK1
Plasmid#220919PurposeNeuronal specific expression of PGK1DepositorInsertPGK1 (PGK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV DJ-1 C106A
Plasmid#220914PurposeExpresses DJ-1 with the C106A mutationDepositorInsertDJ-1 (PARK7 Human)
UseTagsExpressionMammalianMutationC106APromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-DQNAT
Plasmid#198215PurposepJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tagDepositorInsertMBP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PD-DQNAT
Plasmid#198216PurposepJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tagDepositorInsertPD-DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-AQNAT
Plasmid#198211PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_AQNAT
UseTags6x His Tag and AQNAT mutated (non-permissible) gl…ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-MOOR
Plasmid#198212PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tagDepositorInsertsfGFP_MOOR
UseTags6x His Tag and MOOR glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-228)-H6
Plasmid#183779PurposeExpress PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-SU(Z)12(624-900)-H6
Plasmid#183772PurposeExpress of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.DepositorInsertSU(Z)12 residues 624-900 (Su(z)12 Fly)
UseTagsHis tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSunS 1-335
Plasmid#171673PurposeSunS 1-335 expression in E. coliDepositorInsertGlycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30 W96A-GLTP
Plasmid#170734PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertW96A-GLTP (GLTP Human)
UseTagsHisExpressionBacterialMutationW96APromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 mSmoothened NQ4
Plasmid#126407PurposeExpression of Smoothened NQ4 in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationSmo glycosylation site NQ4PromoterAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 mSmoothened NQ3
Plasmid#126406PurposeExpression of Smoothened NQ3 in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationSmo glycosylation site NQ3PromoterAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS315-PGK-SEIPIN2
Plasmid#96987PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.DepositorInsertSEIPIN2 (AT1G29760 Mustard Weed)
UseTagsExpressionYeastMutationPromoterPhosphoglycerate Kinase (PGK) promoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV PGK1-3xHA
Plasmid#220913PurposeExpresses PGK1-3xHADepositorInsertPGK1-3xHA (PGK1 Human)
UseAffinity Reagent/ AntibodyTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
hDAGLa-V5
Plasmid#87676PurposeExpresses human DAGL? protein with intracellular V5 tag located on the C-terminalDepositorInsertHuman Diacylglycerol Lipase Alpha (DAGLA Human)
UseTagsV5 tagExpressionMammalianMutationPromoterT7Available sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MGAT1-GFP
Plasmid#163647PurposeExpresses GFP tagged MGAT1 in mammalian cellsDepositorInsertalpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHM024Env
Plasmid#137691PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only