We narrowed to 3,270 results for: Streptococcus
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Plasmid#155278PurposeLentiviral Cas9 expression with CFP markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9
ExpressionMammalianAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX165-xCas9
Plasmid#140568PurposeExpresses xCas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
MutationA262T R324L S409I E480K E543D M694I E1219VAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK601
Plasmid#110607PurposeBTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseSynthetic BiologyAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330 Rosa26 AscI LH291Gu1 (LH500)
Plasmid#99628PurposePlasmid used to target the pre-modified Rosa26 allele with Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRTags3xFLAGAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3
Plasmid#79613PurposeExpresses dCas9-SH3 in yeast cellsDepositorPublicationInsertSpCas9
TagsSH3 domainExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-NLS-3XHA-NLS
Plasmid#69220PurposeExpresses wild type SpCas9 in mammalian cellsDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268
Plasmid#69224PurposeExpresses R1335K mutant SpCas9 fused to Zif268 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLS and Zif268ExpressionMammalianMutationArginine 1335 is changed to LysineAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIB164
Plasmid#90187PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIB169
Plasmid#90192PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoterDepositorTypeEmpty backboneTagsHis-tag and Strep-tagExpressionBacterialAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
px459 VRER
Plasmid#101716PurposesgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 VRER
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E and T1337R)Available sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580AAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO5d.SFFV.Cas9.P2A.BSD
Plasmid#181981PurposeLentiviral Cas9 expressionDepositorInsertCas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
UseCRISPR and LentiviralAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-(BsaI)_CBh-UN-Cas9
Plasmid#135011PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9DepositorInserthumanized S. pyogenes Cas9
Tags3xFLAG and 126aa domain from HSV-1 UL12 fused to …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the N-termi…PromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-Sth1Cas9
Plasmid#221386PurposeCas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSth1 Cas9
UseCRISPRAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIB168
Plasmid#90191PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only