We narrowed to 18,550 results for: Tec

1. SPLICS Mt-ER- PM Short P2A

   Plasmid

   #164110

   Purpose

   Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP PM- Mt-ER Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCer-4aa-VAMP2

   Plasmid

   #53233

   Purpose

   Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

3. SPLICS Mt-ER- Po Short P2A

   Plasmid

   #164118

   Purpose

   Detect the short Peroxisomes-mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP Mt-ER- Po Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Tight-Puro_Nkx2.2

   Plasmid

   #64840

   Purpose

   A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Nkx2.2 (Nkx2-2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-CLCa

   Plasmid

   #196912

   Purpose

   Visualization of clathrin-coated pit formation in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

   Insert

   Clathrin-Coated light chain
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Tight-Puro_Olig2

   Plasmid

   #64842

   Purpose

   A lentiviral vector for the expression of mouse Olig2 under doxycyline control

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Olig2 (Olig2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL-SFFV.Reporter.RFP657.PAC

   Plasmid

   #61395

   Purpose

   Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos3.2-cortactin

   Plasmid

   #193287

   Purpose

   mammalian transient expression of mEos3.2-cortactin

   Depositor

   Klaus Hahn

   Article

   Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

   Insert

   cortactin (Cttn Mouse)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPLICS Mt-ER- Po Long P2A

   Plasmid

   #164117

   Purpose

   Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP Mt-ER- Po Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-Tight-Puro_ST18

    Plasmid

    #64844

    Purpose

    A lentiviral vector for the expression of mouse ST18 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    ST18 (St18 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. VAMP2-12aa-mCit

    Plasmid

    #53237

    Purpose

    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    12aa-linker and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLVX-Tight-Puro_Nkx6.2

    Plasmid

    #64846

    Purpose

    A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Nkx6.2 (Nkx6-2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX puro TRIM21 C54Y - GFP

    Plasmid

    #116942

    Purpose

    Expression of TRIM21 C54Y -GFP fusion

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 C54Y-GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C54Y

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGS_N_3xFLAG

    Plasmid

    #101654

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, 3xFLAG tag
    

    Use

    Tags

    3xFLAG tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ca-LiPPI-CLAPon1.0 (APEX)

    Plasmid

    #213408

    Purpose

    Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)

    Depositor

    Wenjing Wang

    Article

    Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

    Insert

    MK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pExpreS2-1-C3-PA

    Plasmid

    #175446

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-Protein A and BiP Secretion Peptide

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJA181

    Plasmid

    #128539

    Purpose

    Expresses anti-Prunus dulcis scFv Ab+BirA tag

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6 plus BirA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBL_DK193

    Plasmid

    #156424

    Purpose

    Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (Q57H)
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    Q57H

    Promoter

    Polyhedrin

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-Tight-Puro_Myt1

    Plasmid

    #64845

    Purpose

    A lentiviral vector for the expression of mouse Myt1 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Myt1 (Myt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge