We narrowed to 7,628 results for: ef1a

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AAV-Ef1a-InteinC-CreC

Plasmid

#187615

Purpose

AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

InteinC-CreC

Use

AAV

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mitoGFP-DIO

Plasmid

#174112

Purpose

Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Has Service

AAV2

Insert

mitoGFP (COX8A Human)

Use

AAV

Tags

Cox8 targeting sequence and GFP

Promoter

Ef1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon oG

Plasmid

#131778

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

rabies glycoprotein - oG

Use

AAV; Intrsect

Tags

none

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO_EGFP-GFE3

Plasmid

#79871

Purpose

For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Has Service

AAV1

Insert

GFP-GFE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-CreN-InteinN

Plasmid

#187614

Purpose

AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

CreN-InteinN

Use

AAV

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-Ef1a-OCT4-3xFLAG

Plasmid

#216196

Purpose

Overexpress human OCT4 in HEK 293T cells for use in whole cell extract EMSAs

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

OCT4 (POU5F1 Human)

Use

Lentiviral

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hMLH1dn-Hygro

Plasmid

#195512

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn-P2A-HygroR (MLH1 Human)

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Mutation

Human MLH1 del754-756

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

Plasmid

#89149

Purpose

An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM3D(Gq)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-blast

Plasmid

#167818

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-GCaMP6f

Plasmid

#128315

Purpose

Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1

Insert

GCaMP6f

Use

AAV

Tags

His Tag

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-ER-KLF2-T2A-mCHERRY

Plasmid

#224297

Purpose

Expresses estrogen receptor linked KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

Estrogen Receptor linked KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Tags

Estrogen Receptor

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-VP64_Blast

Plasmid

#192655

Purpose

3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEf1a-goldDn29-P2A-GFP

Plasmid

#247153

Purpose

Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAF0474 Ef1a-Si74_attB acceptor

Plasmid

#193475

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Si74_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEf1a-Dn29-P2A-GFP

Plasmid

#247151

Purpose

Mammalian expression of a human codon optimized wildtype Dn29 recombinase

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a FLAG-CHMP2B-d55-96

Plasmid

#232013

Purpose

Expression of FLAG-tagged CHMP2B with the second alpha helix deleted.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Δ55-96

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAID-EF1a linearizing in pX330

Plasmid

#140610

Purpose

Crispr/Cas9 plasmid for linearization of pAID-EF1a

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

sgRNA for pAID-EF1a plasmid digestion

Use

CRISPR

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC293 - pAAV EF1a V5-synuclein (WT)

Plasmid

#60057

Purpose

An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

alpha-synuclein (SNCA human, Human)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-pMagHigh-FlpC

Plasmid

#180586

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI EF1alpha FLAG/HA-PALI1

Plasmid

#114445

Purpose

Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

PALI1 (LCOR Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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