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Anti-Ankyrin-G (staining) [N106/43R]

Plasmid

#128635

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-G (staining) (Human). Derived from hybridoma N106/43.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Ankyrin-G (staining) (Human) recombinant mouse monoclonal antibody (ANK3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

Plasmid

#58492

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

deleted amino acids P29 to D408 and bearing mutat…

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-MFSD12_STOP

Plasmid

#161106

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD12 (MFSD12 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-V5-SHLD3

Plasmid

#114124

Purpose

Expresses N-terminally tagged V5-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

V5

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 23, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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Ankyrin-G scFv [N106/36]

Plasmid

#199422

Purpose

Mammalian expression of Ankyrin-G scFv. Derived from hybridoma N106/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingAnkyrin-G (Human) (ANK3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXQ1

Plasmid

#101628

Purpose

Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXQ1 (FOXQ1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_MFSD12

Plasmid

#131940

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD12 (MFSD12 Human)

Expression

Mammalian

Available Since

Oct. 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2

Plasmid

#153538

Purpose

AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

EF1α1.1

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5A tail only

Plasmid

#166956

Purpose

Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A (820-1027aa)

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IDH1-R132L

Plasmid

#116411

Purpose

Lentiviral expression of IDH1 R132L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Mutation

R132L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pETM30-ARC105

Plasmid

#133426

Purpose

Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Expression

Bacterial

Mutation

Contains amino acids 5-78 fused to C-terminal of …

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSN879

Plasmid

#190693

Purpose

Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).

Depositor

Shinsuke Niwa

Article

Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

Insert

mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A) (Kif5c, Kif5b, Kif5a Synthetic)

Use

RNAi

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cas9-T2A-STOP-TdT

Plasmid

#126425

Purpose

Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)

Expression

Mammalian

Mutation

alanine-6 of TdT has been changed to a STOP codon

Promoter

pCMV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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DDR1 gRNA (BRDN0001162484)

Plasmid

#78011

Purpose

3rd generation lentiviral gRNA plasmid targeting human DDR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DDR1 (DDR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DDR1 gRNA (BRDN0001162509)

Plasmid

#78012

Purpose

3rd generation lentiviral gRNA plasmid targeting human DDR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DDR1 (DDR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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B52 + CHEK2 sgSTOP

Plasmid

#100712

Purpose

B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti/TO/EYFP-P2A-IDH1(HA)

Plasmid

#122482

Purpose

A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

Depositor

Eric Huang

Article

Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

Insert

isocitrate dehydrogenase 1 (IDH1 Human)

Use

Lentiviral

Tags

HA and P2A

Expression

Mammalian

Promoter

CMV/TO

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-arc105

Plasmid

#133425

Purpose

Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Expression

Bacterial

Mutation

Contains amino acids 1-95 or ARC105 fused to the …

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

Plasmid

#204697

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300 (BRD4, EP300 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

direct fusion of BRD4 to p300

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-AMIGO3-COMP5AP-AviTag-9xHis

Plasmid

#157369

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

AMIGO3 (AMIGO3 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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