Addgene

We narrowed to 6,930 results for: Cad

Showing: 1801 - 1850 of 6930 results

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P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

-

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-10u

Plasmid

#13538

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207R in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pAP1698-4

Plasmid

#105242

Purpose

mammalian eGFP

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBSFI-p110γ

Plasmid

#34892

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110γ (PIK3CG Human)

Use

Adaptor plasmid

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
pGSTag DLC1

Plasmid

#24267

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1 Human)

Use

Tags

Gst

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pDRf1-SweetTrac1

Plasmid

#169083

Purpose

Expression of SweetTrac1 biosensor in yeast

Depositor

Lily S Cheung

Article

Park et al Proc Natl Acad Sci U S A. 2022 Jan 25;11

Insert

SweetTrac1

Use

Tags

Expression

Yeast

Mutation

Promoter

PMA1

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate1-N1

Plasmid

#31870

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-I179N

Plasmid

#12295

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

I179N

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pFLAG-CMV4-ZIKV-NS4B

Plasmid

#178846

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS4B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-U63-QtgRNA2.1-8R

Plasmid

#170514

Purpose

gRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-1m

Plasmid

#13540

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-YPT7

Plasmid

#18847

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT7 (YPT7 Budding Yeast)

Use

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Use

Tags

6xHis-TEV

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AG

Plasmid

#182155

Purpose

Expression of chimeric AG (N-mApple-intron-C-eGFP)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AG

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-GA

Plasmid

#182156

Purpose

Expression of chimeric GA (N-eGFP-intron-C-mApple)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

GA

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24 shRescue

Plasmid

#28139

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V; aa 308 and 309 mutated for shRNA rescue

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
DHRS4

Plasmid

#39071

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DHRS4 (DHRS4 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
KI-eab2-AA

Plasmid

#182157

Purpose

Knockin plasmid with homologous arm for making zMADM-AA zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KI-eab2-GG

Plasmid

#182158

Purpose

Knockin plasmid with homologous arm for making zMADM-GG zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KI-eab2-AG

Plasmid

#182159

Purpose

Knockin plasmid with homologous arm for making zMADM-AG zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KI-eab2-GA

Plasmid

#182160

Purpose

Knockin plasmid with homologous arm for making zMADM-GA zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Phat2-dr-cPla2-C2-mCherry-6H

Plasmid

#187104

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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vector-dendra2 PCDNA

Plasmid

#121134

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 shMTH1-1

Plasmid

#21297

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-1 (NUDT1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pBT366_pattB-Hb9-GFP-pA-FRT5

Plasmid

#52552

Purpose

for PhiC31 integrase-mediated integration of pHb9-GFP

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3512

Plasmid

#47231

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pcdh10a-left (pcdh10a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
pNLS-LSSmKate2-N1

Plasmid

#31871

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-MyD88-P200H

Plasmid

#12288

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P200H

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pRS416 Gal-RNQ1

Plasmid

#18684

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pVM013

Plasmid

#64768

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Nck1 (SH2 mut)

Plasmid

#45893

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 SH2 mutant (NCK1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

in SH2 domain (R308K) and E302K and Q333H

Promoter

CMV

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138318

Purpose

Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SauCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Article

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
2F+NFE2

Plasmid

#24656

Depositor

Yaacov Ben-David

Article

Lu et al Proc Natl Acad Sci U S A. 1994 Aug 30. 9

Insert

p45 NFE2 (Nfe2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1 shMTH1-2

Plasmid

#21298

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-2 (NUDT1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pZS*11-yfp0

Plasmid

#53241

Purpose

express yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)

Depositor

Philippe Cluzel

Article

Subramaniam et al Proc Natl Acad Sci U S A. 2013 Feb 5;110

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtetO1

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW102ParsR-Amp32C

Plasmid

#78637

Purpose

pBW100ParsR carrying 30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA HA HP1 gamma delta C

Plasmid

#24081

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Missing C-terminal 25 amino acids

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pTSA-38

Plasmid

#17328

Depositor

Takeshi Sano

Article

Sano et al Proc Natl Acad Sci U S A. 1995 Apr 11. 9

Insert

streptavidin

Use

Tags

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Promoter

Available since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
pDEST-APIC-Flp1

Plasmid

#112806

Purpose

gateway cloning destination vector to express enhancer driven Flip1 in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-BCB

Plasmid

#127471

Purpose

coding sequence for synthetic zinc finger ZF-BCB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30 NFAT5 DBD

Plasmid

#13629

Depositor

Anjana Rao

Article

Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

Insert

NFAT5 (NFAT5 Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

DNA binding domain: aa 175 to 471

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
Ptrc::RpaA D53A

Plasmid

#105229

Purpose

inducible expression of unphosphorylatable variant of RpaA (D53A)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

rpaA driven by Ptrc promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

D53 mutated to A

Promoter

ptrc

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Thy1.2-Roxed-Cre

Plasmid

#51276

Purpose

Expression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassette

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Roxed-Cre

Use

Tags

Expression

Mutation

Promoter

Thy1.2

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3126

Plasmid

#41300

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nphs2-Left (nphs2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
p6869 pOZ-N puro HA-HPV18 E2

Plasmid

#35134

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV18 E2 (E2 HPV18)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
hrGFP-BATS

Plasmid

#30496

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 Apr 25. (

Insert

Barkor (ATG14 Human)

Use

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

413-492 aa

Promoter

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only

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