We narrowed to 59,840 results for: ica;
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Plasmid#113934PurposeAlso termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFPDepositorInsertLOV2 and Stim1 polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
piGECI-N1
Plasmid#160421PurposeCytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECIDepositorInsertiGECI
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-1P-WPRE
Plasmid#202613PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSynDepositorInsertJEDI-1P
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSc-humanADAR2
Plasmid#171106PurposeExpresses human ADAR2 in yeast.DepositorInserthuman ADAR2 (ADARB1 Human)
UseTagsHis12_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
IMS-HyPer2
Plasmid#60248PurposeGenetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.DepositorInsertIMS-HyPer2
UseTagsMitochondrial intermembrane space targeting signa…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-JEDI-1P-WPRE
Plasmid#202615PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIDepositorInsertJEDI-1P
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-RexYFP
Plasmid#141070PurposeBacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratioDepositorInsertRexYFP
UseTags6xHisExpressionBacterialMutationchanged Leucine 169 to Proline, changed Tyrosine …PromoterT5Available sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FRISZ-TM
Plasmid#176886PurposeAAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoterDepositorInsertIg kappa-FRISZ-PDGFR TM
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-JEDI-1P-WPRE
Plasmid#202616PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAPDepositorInsertJEDI-1P
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
piGECI-NES-N1
Plasmid#160422PurposeExpression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequenceDepositorInsertiGECI-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
YEpURA-CUP1-HBTubiquitin
Plasmid#26900DepositorInsertHBT-tagged ubiquitin
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v3
Plasmid#79539PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 3DepositorInsertAmrose v3
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v2
Plasmid#79538PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 2DepositorInsertAmrose v2
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
UseTags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clomeleon
Plasmid#203723PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentrationDepositorInsertcyan fluorescent protein linked yellow florescent protein
UseTagsExpressionBacterial and MammalianMutationCyan Fluorescent Protein, Topaz Fluorescent Prote…PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1
Plasmid#32466DepositorInsertG-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…ExpressionBacterialMutationGCaMP3 K119I/L173Q/S404G/E430VPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lapronic/pRSETB
Plasmid#140755PurposeFRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate RatioDepositorInsertLapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-FRISZ
Plasmid#176887PurposeFar-red fluorescent zinc indicator FRISZ in pDisplayDepositorInsertFRISZ
UseTagspDisplay backbone elements for export and membran…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.1
Plasmid#32467DepositorInsertG-GECO1.1
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only