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pCIS.MTRIA-NPY

Plasmid

#184613

Purpose

For expression of a fluorescent sensor for neuropeptide Y in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NPY

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-Tubulin-C-18

Plasmid

#57432

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Tubulin (TUBA1B Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA5/FRT-ChemoG1

Plasmid

#193806

Purpose

CMV overexpression of ChemoG1 in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Tags

10xHis

Expression

Mammalian

Mutation

EGFP: A206K

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDest-623 (attR4-attR3)

Plasmid

#161878

Purpose

Multisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-SST

Plasmid

#184619

Purpose

For expression of a fluorescent sensor for somatostatin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-SST

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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Clathrin-LSSmGFP

Plasmid

#186537

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to clathrin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Clathrin

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSA12

Plasmid

#186546

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPD159 dsRedExpress2-ZFi43

Plasmid

#138799

Purpose

Constitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

dsRedExpress2-ZFi43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJB37

Plasmid

#202602

Purpose

Arabinose inducible expression vector expressing retron Rcat-Sen2

Depositor

Athanasios Typas

Article

Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

Insert

RcaT-Sen2 toxin

Expression

Bacterial

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

Plasmid

#173453

Purpose

Contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E15: attB4-EF1p>-attB1r

Plasmid

#162920

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8381

Plasmid

#126915

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and tHMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-GST-eIF4G1-His6

Plasmid

#37231

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF4631 (TIF4631 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

tac

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pDREV-2

Plasmid

#26752

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

H2B-Venus CDS (H2BC3 Human)

Use

Cre/Lox; Flp/frt; dre/rox

Tags

Venus

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pEPSW1KN0034

Plasmid

#196178

Purpose

MoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

CaMV35S::TMV::LucF::flag::35sT

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLBC0151

Plasmid

#172796

Purpose

Level 0 module containing CT_TurboID

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

TurboID with 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AKAP1-mCherry-LOVPLD

Plasmid

#219558

Purpose

mitochondria-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

AKAP1-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKL12-GCaMP6f-pHuji bb118

Plasmid

#158981

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-pHuji

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833 a…

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-miR-10b sponge

Plasmid

#25043

Depositor

Bob Weinberg

Article

Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

Insert

miR-10b sponge

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only