We narrowed to 59,588 results for: ran

1. pAWH-largeT

   Plasmid

   #133895

   Purpose

   Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   LargeT
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3_altPKA-Cα-mEGFP

   Plasmid

   #114132

   Purpose

   Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   18747 (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHelper-T7IS1

   Plasmid

   #140631

   Purpose

   Expresses ShCAST under the T7 promoter and an empty sgRNA

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_EF1a-CasRx-msfGFP-2A-Blast

   Plasmid

   #226010

   Purpose

   CasRx protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   CasRx RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cerulean-GalT

   Plasmid

   #11930

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Available Since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-7aa-ActA-pEGFP-C1

   Plasmid

   #177406

   Purpose

   To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

   Depositor

   David Andrews

   Article

   Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL0812 (pCRISPR(BbsI)_Vch)

   Plasmid

   #200898

   Purpose

   Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCRISPR (entry)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

   Plasmid

   #113890

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. amilGFP chromoprotein promoter-less

   Plasmid

   #118006

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, amilGFP
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOP/SSAT

    Plasmid

    #140291

    Purpose

    Expresses human SSAT in bacterial cells

    Depositor

    Saverio Bettuzzi, Gaetano Marverti

    Article

    Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

    Insert

    SSAT1 (SAT1 Human)
    

    Expression

    Bacterial

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. L1-1180

    Plasmid

    #13265

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Expression

    Bacterial

    Mutation

    Truncated after RSLE region

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pCW57.1_V5_mNeonGreen_FLAG_C

    Plasmid

    #218073

    Purpose

    RESOLUTE cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-mNeonGreen-V5

    Available Since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE MDER

    Plasmid

    #13494

    Depositor

    Stephen Tapscott

    Article

    Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

    Insert

    MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    full length MyoD was fused to aa282-595 of Esr1. …

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBR322-MCK1.35lux

    Plasmid

    #53368

    Purpose

    expresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancer

    Depositor

    Josephine Nalbantoglu

    Article

    Larochelle et al Gene Ther. 1997 May;4(5):465-72.

    Insert

    muscle creatine kinase promoter/enhancer 1.35kb
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid

    #100018

    Purpose

    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpsfGFP-LSSmOrange
    

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF005-sgRNA-shuffle-in

    Plasmid

    #104441

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF004-sgRNA-shuffle-in

    Plasmid

    #104440

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    U6 promoter

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT3024

    Plasmid

    #122542

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_mt::CemOrange2
    

    Tags

    MLSLRQSIRFFKPATRTLCSSRTLL

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_GW_Dest

    Plasmid

    #86955

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRB-mCherry-EEA1

    Plasmid

    #222419

    Purpose

    To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    EEA1 (EEA1 Human)
    

    Tags

    FRB-mCherry

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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