161,424 results

1. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

   Plasmid

   #85428

   Purpose

   Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   human IL-12
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1036 pcDNA3 Myr HA Akt1

   Plasmid

   #9008

   Purpose

   Mammalian expression of human Akt1 with myr, HA tags

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   HA and myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR4-FKBP12DD N-term A (B3-1)

   Plasmid

   #17414

   Purpose

   Gateway entry vector for Degron, N-term, frame A

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   FKBP12DD (L106P)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. His6_MBP_FMRP_P451S

   Plasmid

   #198693

   Purpose

   Expression of human FMRP protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FMR1 (FMR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed proline 451 to serine

   Promoter

   T7

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HSC1-GiP

   Plasmid

   #58254

   Purpose

   This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1-alpha

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Desmoglein2-C-18

   Plasmid

   #55031

   Purpose

   Localization: Desmosomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Desmoglein2 (DSG2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. hTLR3-pcDNA3

   Plasmid

   #32712

   Depositor

   Saumen Sarkar

   Article

   Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

   Insert

   TLR3 (TLR3 Human)
   

   Use

   Tags

   FLAG and Preprototrypsin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pN3-Sp1FL

   Plasmid

   #24543

   Depositor

   Guntram Suske

   Insert

   Sp1FL (SP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MPZ(P0)promoter pGL3-TATA

    Plasmid

    #21259

    Depositor

    Jerry Crabtree

    Article

    Kao et al Science. 2009 Jan 30. 323(5914):651-4.

    Insert

    myelin protein zero promoter (Mpz Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-bARGSer-AxeTxe

    Plasmid

    #192473

    Purpose

    Second-generation bacterial acoustic reporter gene derived from Serratia

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Inserts

    bARGSer
    AxeTxe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    the gene Ser39006_001280 was deleted from the clu…

    Promoter

    Native promoter from Enterococcus faecium and pBAD

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. dGFP-Rab27a

    Plasmid

    #170862

    Purpose

    Mammalian expression of dGFP-Rab27a.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Rab-27a (RAB27A Human)
    

    Use

    Tags

    dEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFSy(1.1)GW

    Plasmid

    #27232

    Depositor

    Pavel Osten

    Article

    Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-Caspase 1

    Plasmid

    #41552

    Depositor

    Kate Fitzgerald

    Article

    Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate-early enhancer/promoter

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJFRC81-10XUAS-IVS-Syn21-GFP-p10

    Plasmid

    #36432

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLminP_Luc2P_RE31

    Plasmid

    #90373

    Purpose

    Hippo - TEAD1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Hippo

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3puro-mGFP

    Plasmid

    #225446

    Purpose

    Express mEGFP-fusion protein of interest

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CL20_mEGFP_NUP98_NSD1

    Plasmid

    #205873

    Purpose

    Express mEGFP-tagged fusion protein, NUP98_NSD1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    NUP98_NSD1 (NUP98, NSD1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PVS10

    Plasmid

    #104398

    Purpose

    polycistronic vector for expression of E. coli core RNAP

    Depositor

    Irina Artsimovitch

    Article

    Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

    Insert

    rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBMCL1

    Plasmid

    #178203

    Purpose

    An improved vector for BacMam system

    Depositor

    Lei Chen

    Article

    Guo et al Biochem Biophys Res Commun. 2022 Feb 26;

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pSC-humanADAR1

    Plasmid

    #198235

    Purpose

    Expresses human ADAR1 in yeast

    Depositor

    Brenda Bass

    Article

    Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

    Insert

    human ADAR1 (ADAR Human)
    

    Use

    Tags

    His10_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-smURFP-StagRFP-EKAR-EV

    Plasmid

    #173019

    Purpose

    Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    smURFP-StagRFP-EKAR-EV
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-PIK3CA

    Plasmid

    #116771

    Purpose

    Lentiviral expression of PIK3CA

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pemiRFP670-tubulin

    Plasmid

    #136573

    Purpose

    Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP670-tubulin
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.3_CoV1_D28

    Plasmid

    #170447

    Purpose

    Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    SARS-CoV-1 spike D28 (S SARS-CoV-1)
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    last 28aa deletion in c-terminal tail

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid

    #104909

    Purpose

    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

    Plasmid

    #85717

    Purpose

    Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Sp dCas9
    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO and U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5/FRT/TO_HaloTag7_LamB1

    Plasmid

    #175528

    Purpose

    Expression of HaloTag7 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag7-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge