We narrowed to 19,528 results for: MUT

Showing: 7521 - 7540 of 19528 results

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EGFP-P2A_BAF_G47E

Plasmid

#101774

Purpose

Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

G47E mutation

Promoter

EF1a

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

Plasmid

#233629

Purpose

Expression of bicistronic reporter with mutated TOP motif

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)

Use

Luciferase

Mutation

AGAAGG in TOP motif

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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2xMARS

Plasmid

#205233

Purpose

Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Insert

two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)

Tags

Nuclear Export Sequence and mScarlet-i

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1_RAF1 (51-131)

Plasmid

#119218

Purpose

purification of GST-RAF1 RBD from E. coli

Depositor

Helen Morrison

Article

Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

Insert

RAF1 aa 51-131 (RAF1 Human)

Expression

Bacterial

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pF142 pAcGFP1 SOD1 A4V

Plasmid

#26403

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 T18N

Plasmid

#47897

Purpose

Expresses myc tagged Miro2 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 T18N (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 A13V

Plasmid

#47896

Purpose

Expresses myc tagged Miro2 A13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 A13V (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

A13V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pJM656

Plasmid

#53086

Purpose

A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Lactaherin (Mfge8 Mouse)

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

deleted native signal sequence, deleted C-termina…

Promoter

hsp-16.41

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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GFP KNL1 wt hardened and RVSF->AAAA

Plasmid

#45225

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Mutation

RVSF mutated to AAAA

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
pShuttle-EGFP-CLASP2 340-1362 9xS/A

Plasmid

#24373

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) 9xS/A (CLASP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pInducer20 TIFA T9A

Plasmid

#231590

Purpose

TIFA gene mutated in position T9A (mutation on phosphorylation site) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

TIFA (TIFA Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

T9A

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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PCMV-Tag4A-MMP8 (E138Q)

Plasmid

#29549

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E138Q

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

Plasmid

#175256

Purpose

SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pegRNA-HEK3_del1-5-NGG

Plasmid

#185478

Purpose

pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

HEK3_del1-5-NGG pegRNA

Expression

Mammalian

Promoter

U6

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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cpVenus-Gγ2

Plasmid

#69626

Purpose

The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

cp173Venus-Ggamma2 (GNG2 Synthetic)

Tags

ciruclar permutated Venus (cp173V)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET-His6-SUMO-MproC145A

Plasmid

#172653

Purpose

Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

Depositor

Stephen Harrison

Article

MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

Insert

SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis Tag and SUMO

Expression

Bacterial

Mutation

Catalytic Cysteine 145 mutated to Alanine

Promoter

T7

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-Cep290

Plasmid

#27380

Depositor

Joseph Gleeson

Article

Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

Insert

Cep290 (Cep290 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

please see depositor comments below

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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HDM_Spikedelta21_sinobiological_B.1.617.2

Plasmid

#204149

Purpose

Expression plasmid for SARS-CoV-2 Delta variant spike

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

SARS-CoV-2 Delta variant spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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TPC2(N257A)-mCherry

Plasmid

#135186

Purpose

Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Mutation of asparagine257 to alanine

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hPhyBY276H-mCherry-HRasCT

Plasmid

#100540

Purpose

Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)

Tags

mCherry fused with HRas C-terminus for plasma mem…

Expression

Mammalian

Mutation

Human codon optimized PhyB (1-908 aa), Y276H muta…

Promoter

CAG promoter

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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