We narrowed to 1,335 results for: piggyBac

1. pAS_4xMma PylT_GFP 150TAG 6xHis

   Plasmid

   #140015

   Purpose

   Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-PtH2B

   Plasmid

   #133390

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   No promoter

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BII-CPspaH2B

   Plasmid

   #133385

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. nos-Cas9.874Z1

   Plasmid

   #112685

   Purpose

   Express hSpCas9 under nanos promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   nanos

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC103-PBNeo-U6-sgRNA-Expression

   Plasmid

   #48228

   Purpose

   sgRNA expression on PiggyBac vector with Neo-selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-GFP1-10-HygR

   Plasmid

   #223675

   Purpose

   To check the split GFP signals in mitochondria (1)

   Depositor

   Juan Melero-Martin

   Article

   Lin et al Nature. 2024 May;629(8012):660-668. doi:

   Insert

   GFP1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_TRE_sg30-PDGFR_EF1a_mCherry

   Plasmid

   #186309

   Purpose

   Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg30-PDGFR
   mCherry
   

   Use

   Tags

   His, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

   Plasmid

   #167901

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-EZH2

   Plasmid

   #222528

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAM1.16_PB-Cx43_S368A-PGK-Blast

    Plasmid

    #205561

    Purpose

    Express human Cx43 with S368A mutation

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Cx43
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    S368A

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-GFP11x7-HygR

    Plasmid

    #223676

    Purpose

    To check the split GFP signals in mitochondria (2)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP11x7
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. KA0637_pPBCAG-rtTAM2-IN

    Plasmid

    #124166

    Purpose

    piggyBac-based expression of rtTA-M2

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    rtTAM2-IRES-Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid

    #186310

    Purpose

    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg61-PDGFR
    eGFP
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. KA0718_pPB-TREtight-cHA-IRESVenus

    Plasmid

    #124171

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ubiq-Cas9.874W

    Plasmid

    #112686

    Purpose

    Express hSpCas9 under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    Ubiquitin-63E promoter

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. vYM140_PB-pEFa1-iaaM(P.s)-Hygro

    Plasmid

    #160043

    Purpose

    Expressing iaaM for converting L-Trp to IAM

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Insert

    iaaM (iaaM )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. BII-ChBtW-iresFUCCI

    Plasmid

    #133371

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. XLone-Cas9-2A-GFP

    Plasmid

    #170159

    Purpose

    Express Cas9 and GFP upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    Cas9
    

    Use

    Synthetic Biology; Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G promoter

    Available Since

    July 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB_TRE_Z18-PDGFR_EF1a_eGFP

    Plasmid

    #186304

    Purpose

    Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z18-PDGFR
    eGFP
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

    Plasmid

    #160045

    Purpose

    For expressing the active auxin pump PIN2

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Insert

    PIN2
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    pEF1s

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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