Addgene

We narrowed to 23,367 results for: Nov;

Showing: 11461 - 11490 of 23367 results

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pET29a-hNABP1

Plasmid

#128306

Purpose

Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178576

Purpose

AAV vector for CK(0.4)::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

AAV

Tags

Expression

Mutation

Promoter

CamKIIa

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVTREGCaMP6f

Plasmid

#97410

Purpose

Amplified expression of GCaMP6f in combination with TET driver

Depositor

Tetsuo Yamamori

Article

Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE3

Available since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-AID-BubR1

Plasmid

#47330

Purpose

for plasmid integration using the Flp-In system. AID at N-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)

Use

Tags

AID and GFP

Expression

Mammalian

Mutation

siRNA resistant

Promoter

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Ubc13-WT

Plasmid

#43938

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-LIN-41

Plasmid

#52716

Purpose

retroviral expression of human LIN-41 / TRIM71

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHY002_Strep_Avi_hsDCAF15

Plasmid

#174479

Purpose

Expresses DCAF15 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DCAF 15 (DCAF15 Human)

Use

Tags

StrepII-Avi-TEV

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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VN-beta Synuclein

Plasmid

#89483

Purpose

expresses human beta Synuclein fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

Insert

SNCB (SNCB Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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LV

Plasmid

#202004

Purpose

Empty lenti backbone

Depositor

Nikhil Joshi

Article

Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

Plasmid

#205766

Purpose

Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa

Use

AAV

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTF023_His_hsDDA1

Plasmid

#174480

Purpose

Expresses DDA1 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DDA1 (DDA1 Human)

Use

Tags

His-TEV

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAIL-R2

Plasmid

#61383

Purpose

Expresses TRAIL receptor 2

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 2 (TNFRSF10B Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18

Plasmid

#214317

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG PIK3CA E545K

Plasmid

#73055

Purpose

Human PIK3CA E545K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

PIK3CA (PIK3CA Human)

Use

Tags

Expression

Mammalian

Mutation

E545K

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA_MS2_neo

Plasmid

#118650

Purpose

To clone sgRNA for activation dCas9

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-tdTomato

Plasmid

#107714

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-tdTomato

Use

AAV

Tags

KA2 and tdTomato

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10-Fc-IG

Plasmid

#183722

Purpose

Expresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Use

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-shPKM2_4

Plasmid

#42516

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

shRNA-PKM2 (PKM Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Ubc13-C87A

Plasmid

#43939

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

C87A

Promoter

Available since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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E. coli expression: Z-lock cofilin dark state mutant

Plasmid

#141135

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Bacterial

Mutation

D3 deletion, I32F in Zdk2

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(A)

Plasmid

#87913

Purpose

Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499A

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP73_sgRNA-1

Plasmid

#88855

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_DeltaN24

Plasmid

#214327

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Deletion of first 24 amino acids

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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