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We narrowed to 7,747 results for: pac

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Showing: 7681 - 7700 of 7747 results

  4. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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  8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

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  10. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  11. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Available Since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132536-plasmid-map-sequence-id-270901

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  12. Mobius Assembly Vector Toolkit

    Plasmid Kit
    #1000000134
    Purpose
    Mobius Assembly is a Golden Gate-based cloning framework which renders both high cloning capacity and vector toolkit simplicity.
    Depositor
    Naomi Nakayama
    Available Since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids designed to restore prototrophy and test metabolic capacity in common S. cerevisiae laboratory strains.
    Depositor
    Markus Ralser
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. Oxford Drosophila melanogaster Genome-wide CRISPR Library

    Pooled Library
    #64750
    Purpose
    Library designed for genomic targeting and screening in Drosophila cells. All possible gRNA target sites within the shared exonic regions on either DNA strand of the Drosophila genome were computed.
    Depositor
    Ji-Long Liu
    Article
    Bassett et al J Genet Genomics. 2015 Jun 20;42(6):301-
    Expression
    Insect
    Species
    Drosophila melanogaster, Streptococcus pyogenes, and Synthetic
    Use
    CRISPR
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. Diatom uLoop Assembly Kit

    Plasmid Kit
    #1000000238
    Purpose
    Universal Loop (uLoop) assembly system for efficient and cross-kingdom DNA assembly and to build conjugatable episomes using diatom promoters and terminators.
    Depositor
    Christopher Dupont
    Available Since
    Feb. 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts.
    Depositor
    Diego Orzaez
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    Collection of MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly.
    Depositor
    Daniel Voytas
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 7681 - 7700 of 7747 results