178,399 results
-
Plasmid#134919PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
ExpressionMammalianPromoterCAGAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT3-EF1A-MYC-IRES-lucOS
Plasmid#129776PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339DepositorInserthuman MYC (MYC Human)
TagsOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…ExpressionMammalianPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MTK3_038
Plasmid#123752PurposeEncodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK systemDepositorInsertR-FlincA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-WT
Plasmid#133753PurposeWild type human TDP-43 in pLVX-Puro lentiviral vectorDepositorAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmNeonGreenHO-G
Plasmid#127912Purposea mammalian expression backbone vector for humanized mNeonGreenDepositorInserthumanized mNeonGreen
TagsmNeonGreenExpressionMammalianAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CDH5-AbR-GFP
Plasmid#122970PurposeLenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.DepositorInsertsCDH5p
CopGFP
Zeocin
UseLentiviralExpressionMammalianMutationNAAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5
Plasmid#124362PurposeOverexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)DepositorInsertTornado-F30-Broccoli-aptNFkB#5
ExpressionMammalianPromoterU6+27Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FU-H2B-GFP-IRES-Puro
Plasmid#69550PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.DepositorInsertHistone 2B - green fluorescent protein fusion
UseLentiviralTagsGFP fusionAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-EZH2
Plasmid#116738PurposeLentiviral expression of EZH2DepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCCLc-MND-A0201-SABR-Backbone
Plasmid#119050Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-Sox10
Plasmid#115242PurposeLentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)DepositorInsertSOX10 (SOX10 Human)
UseLentiviralAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GATA3-pCDNA3.1
Plasmid#115271PurposeExpresses human GATA3 in mammalian cellsDepositorAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZS165 CRISPEY HH-HDV NotI
Plasmid#114451PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsExpressionYeastPromoterScGAL7pAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
Plasmid#115267PurposeExpresses LbuCas13a from a SUMO-tagged bacterial expression constructDepositorInsertLbuCas13a
TagsSUMOExpressionBacterialAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-dLight1.2
Plasmid#111068PurposeTo generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoterDepositorHas serviceAAV1 and AAV5InsertdLight1.2
UseAAVTagsFlag tagAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pR_LG lenti-(empty)-del-CTS
Plasmid#112895PurposeNon-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid librariesDepositorPublicationTypeEmpty backboneUseLentiviralPromoternoneAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediatePromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
VEGFR2-mCh
Plasmid#108854PurposeEncodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hSyn1-SIO-stGtACR2-FusionRed
Plasmid#105677PurposeExpresses a soma-targeted GtACR2 in Cre-expressing neuronsDepositorHas serviceAAV Retrograde, AAV1, and AAV5InsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterhSyn1Available sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG G-CEPIA1er
Plasmid#105012PurposeER calcium sensorDepositorInsertG-CEPIA1er
ExpressionMammalianPromoterpCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by