285,820 results

1. eSrtA(4S-9) pET29b

   Plasmid

   #75146

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N98D S102C A104V A118T F122A K134R F144L I182V E1…

   Promoter

   T7

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-UPEmS-tevo

   Plasmid

   #228894

   Purpose

   Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEB31

   Plasmid

   #51295

   Purpose

   Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Rice snoRNA U3 and dual 35S promoter

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRGEB31

   Plasmid

   #51295

   Purpose

   Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Rice snoRNA U3 and dual 35S promoter

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (AAV9)

   Viral Prep

   #100838-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (#100838). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   Syn

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

8. Ac5-STABLE2-neo

   Plasmid

   #32426

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Insert

   Flag-mCherry-T2A-GFP-T2A-neo
   

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ac5-STABLE2-neo

   Plasmid

   #32426

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Insert

   Flag-mCherry-T2A-GFP-T2A-neo
   

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GRAB_DA1h (AAV9)

    Viral Prep

    #113050-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_DA1h (#113050). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_DA1h plasmid DNA. Synapsin-driven expression of GRAB-DA1h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

    Promoter

    Syn

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

11. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid

    #79601

    Purpose

    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP-Zeb1 3'UTR
    

    Use

    Lentiviral

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid

    #79601

    Purpose

    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP-Zeb1 3'UTR
    

    Use

    Lentiviral

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.iAChSnFR

    Plasmid

    #137955

    Purpose

    Expresses iAChSnFR under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Has Service

    AAV1 and AAV9

    Insert

    iAChSnFR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG.iAChSnFR

    Plasmid

    #137955

    Purpose

    Expresses iAChSnFR under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Has Service

    AAV1 and AAV9

    Insert

    iAChSnFR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL1 sortase

    Plasmid

    #106288

    Purpose

    Expresses sortase in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    sortase A pentamutant
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1 sortase

    Plasmid

    #106288

    Purpose

    Expresses sortase in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    sortase A pentamutant
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AICSDP-10:LMNB1-mEGFP

    Plasmid

    #87422

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. AICSDP-10:LMNB1-mEGFP

    Plasmid

    #87422

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. sUPRa

    Plasmid

    #223242

    Purpose

    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

    Depositor

    Colin Akerman

    Article

    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

    Insert

    sUPRa
    

    Use

    AAV

    Promoter

    Custom

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-HA-PD-L1

    Plasmid

    #121492

    Purpose

    Expression of human PD-L1 WT in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 (CD274 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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