Addgene

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pRSpuro shID3#1

Plasmid

#19163

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3/flag-pBirA(1-G78)-FRB

Plasmid

#154447

Purpose

expresses pBirA(1-G78)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-G78)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRShygro shID3#1

Plasmid

#19164

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pEN_TGmiR_Gb2-K

Plasmid

#25766

Purpose

Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G13-G12

Plasmid

#25742

Purpose

Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 and 12 miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G13

Plasmid

#25741

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_TmiR_Gb2-K

Plasmid

#25764

Purpose

Entry vector with TRE promoter driving mouse G beta 2 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD1

Plasmid

#210264

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD1

Promoter

CMV/TetO2

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 D394R

Plasmid

#210265

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N1

Plasmid

#210268

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N1 (aa 208-419) siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-N1, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N2

Plasmid

#210269

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N2 (aa 261-419) siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-N2, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 D394R

Plasmid

#210267

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 wt

Plasmid

#210266

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Use

Tags

mCherry

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD2

Plasmid

#210263

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD2

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 wt

Plasmid

#210262

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-K283)-FRB

Plasmid

#154451

Purpose

expresses flag-pBirA(1-K283)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-K283)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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FR-E01

Bacterial Strain

#118727

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Bacterial Resistance

None

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only
pBACH2.2.0-gDNA

Plasmid

#176375

Purpose

Expresses spCas9 and gRNA sequence targeting BACH2

Depositor

Kevin White, Barbara Stranger

Insert

BACH2 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSOX2.1.0-gDNA

Plasmid

#176382

Purpose

Expresses spCas9 and gRNA sequence targeting SOX2

Depositor

Kevin White, Barbara Stranger

Insert

SOX2 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSOX11.1.0-gDNA

Plasmid

#176378

Purpose

Expresses spCas9 and gRNA sequence targeting SOX11

Depositor

Kevin White, Barbara Stranger

Insert

SOX11 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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