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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,979 results for: NUC

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Showing: 7801 - 7820 of 12979 results

  5. p420-3

    Plasmid
    #71257
    Purpose
    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Article
    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.
    Insert
    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    GM-CSF minimal
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71257-plasmid-map-sequence-id-131889

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  6. pHR-pSFFV-TAF15N-mCherry-sspB

    Plasmid
    #122436
    Purpose
    Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.
    Depositor
    Clifford Brangwynne
    Article
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    TAF15 (TAF15 Human)
    Use
    Lentiviral
    Tags
    mCherry and sspB
    Expression
    Mammalian
    Mutation
    Contains only amino acids 1-208
    Promoter
    SFFV
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    122436-plasmid-map-sequence-id-243727

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  13. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92114-plasmid-map-sequence-id-197615

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  14. pFRLuc-CoV2-delStem1

    Plasmid
    #177616
    Purpose
    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    3-nucleotide (UAC) deletion in Stem 1
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177616-plasmid-map-sequence-id-350042

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  15. pTU422

    Plasmid
    #178982
    Purpose
    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)
    Depositor
    Stan Brouns
    Article
    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353
    Inserts
    gRAMP
    CRISPR array
    Tags
    Strep-Tag II SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178982-plasmid-map-sequence-id-354261

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Showing: 7801 - 7820 of 12979 results