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pCDNA3-Flag-Tpr

Plasmid

#60882

Purpose

Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

Depositor

Vinay Nandicoori

Article

Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

Insert

Tpr (TPR Human)

Expression

Mammalian

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9 T186A

Plasmid

#28101

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Contains a T186A mutation in Cdk9

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pCMX IkB alpha M

Plasmid

#12329

Depositor

Inder Verma

Article

Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

Insert

IkB alpha M (Nfkbia Mouse)

Expression

Mammalian

Mutation

Dominant-negative mutant. Many signal transductio…

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP1

Plasmid

#72292

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP1 (FNIP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRN3P-HA-Suv4-20h1

Plasmid

#86689

Purpose

For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)

Tags

HA-tag

Promoter

T3

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Rfx4

Plasmid

#61544

Purpose

Expresses Rfx4 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Rfx4 (Rfx4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pCEP4-hKCNE1

Plasmid

#53050

Purpose

Expresses beta subunit for IKs channel in mammalian cells

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_NDG

Plasmid

#26820

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-NDGFP-3'UTR

Expression

Mammalian

Mutation

nuclear destabilized eGFP

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
Rab8WT(Flag) in pcDNA3.1neo

Plasmid

#46783

Purpose

expression of WT Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A (RAB8A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 Y42A/M43A

Plasmid

#19940

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 Y42A/M43A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a SKP1 binding mutant

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
N-Myc-53BP1 D1521A pLPC-Puro

Plasmid

#19837

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Mutation

changed Asp1521 to Ala

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only
psigma1-EGFP

Plasmid

#53611

Purpose

Expresses sigma1 of adaptor protein of AP1

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

sigma 1 (Ap1s1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h2mut

Plasmid

#86692

Purpose

For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)

Tags

HA

Mutation

Mutated asparagine 273 to cysteine 276 (NHDC) int…

Promoter

T3

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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MSP2292

Plasmid

#70707

Purpose

Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

T7

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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1127-DP NTP wild type

Plasmid

#32398

Depositor

Kathleen Green

Article

Kowalczyk et al J Cell Biol. 1997 Nov 3;139(3):773-84.

Insert

Desmoplakin (DSP Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains only the first 584 aa

Promoter

CMV

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
Prolactin-CD4d3+4-bio

Plasmid

#73123

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Prolactin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

PRL (PRL Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 delta 610-615

Plasmid

#19941

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta 610-615 (CUL1 Human)

Tags

HA

Expression

Mammalian

Mutation

CUL1 with a ROC1 binding mutant

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG SMAD4 neo

Plasmid

#83273

Purpose

Lentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 delta C22

Plasmid

#19942

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta C22 (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

Mutation of 22 amino acids from the C terminus

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
CANE-LV envelope

Plasmid

#86666

Purpose

pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus

Depositor

Fan Wang

Article

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

CANE-LV envelope

Use

Lentiviral, Mouse Targeting, and Synthetic Biolog…

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only
pTrc99A-Rat FABP1

Plasmid

#13577

Depositor

Martin Scanlon

Article

Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

Insert

Rat liver fatty acid binding protein (Fabp1 Rat)

Expression

Bacterial

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only
pLYS1-EMRE/RNAi resistant

Plasmid

#50056

Purpose

Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

EMRE-RNAi resistant (SMDT1 Human)

Expression

Mammalian

Mutation

RNAi resistant, silent mutant

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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PatoL-OG241

Plasmid

#72841

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoL

Expression

Bacterial

Promoter

PatoL

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-HA

Plasmid

#27765

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

HA

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-EVE-MM

Plasmid

#22994

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A, S463E, S465E

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pBbE1k-3

Plasmid

#71722

Purpose

tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

Depositor

Taek Soon Lee

Article

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Inserts

dihydropterine reductase (QDPR Human)
pterin-4a-carbinolamine dehydratase (PCBD1 Human)

Promoter

pTrc

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBJ5 BRG1 DN

Plasmid

#17874

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg DN (SMARCA4 Human)

Expression

Mammalian

Mutation

dominant negative

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
HR-TERT-promotor

Plasmid

#71398

Purpose

Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Promoter

Endogenous TERT promotor

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA5/FRT/TO-FNTA-FLAG

Plasmid

#86858

Purpose

To express FNTA with a FLAG tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

FLAG

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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