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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 26,986 results for: VIT

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Showing: 7841 - 7860 of 26986 results

  3. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138566-plasmid-map-sequence-id-274798

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  6. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

    Plasmid
    #231595
    Purpose
    Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter
    Depositor
    Marvin Tanenbaum
    Article
    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.
    Insert
    Kozak sequence-Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
    Expression
    Mammalian
    Available Since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    231595-plasmid-map-sequence-id-459554

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  16. pET30a-SSDH

    Plasmid
    #239573
    Purpose
    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Article
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239573-plasmid-map-sequence-id-476594

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  19. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65206-plasmid-map-sequence-id-490956

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Showing: 7841 - 7860 of 26986 results