161,492 results

1. pLViP-LifeAct-iRFP670

   Plasmid

   #229699

   Purpose

   Lentiviral expression of LifeAct-iRFP670 in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   Synthetic construct LifeAct-mTurquoise gene
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPCX mito roGFP2-Orp1

   Plasmid

   #64992

   Purpose

   Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEGFP-Rac1

   Plasmid

   #83950

   Purpose

   Rac1 FLIM donor for dynamic imaging of GTPase activity

   Depositor

   Ryohei Yasuda

   Article

   Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

   Insert

   ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5068 pGEX-6P-1 Brd4 full-length

   Plasmid

   #14447

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (BRD4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA Seh1L pRK5

   Plasmid

   #46331

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Seh1 (SEH1L Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYHY3

   Plasmid

   #216109

   Purpose

   aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

   Depositor

   Shannon Sirk

   Article

   Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

   Insert

   NanoLuc
   

   Use

   Synthetic Biology; E. coli-bacteroides shuttle ve…

   Tags

   3xFLAG tag and BT3630 signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   P1TDP-A21

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-ccdB-Nanoluc

   Plasmid

   #87067

   Purpose

   Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Use

   Tags

   Nanoluc luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CHRM2-DuET

   Plasmid

   #213208

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CHRM2 (CHRM2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-Opto-zFGFR1a

   Plasmid

   #232639

   Purpose

   Zebrafish FGFR1a receptor kinase domain fused to VfLOV

   Depositor

   Katherine Rogers

   Insert

   zFGFR1a kinase domain + VfLOV domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. HiBiT-FZD2

    Plasmid

    #195840

    Purpose

    Expresses human FZD2 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD2 (FZD2 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSecUAG_UTu1D

    Plasmid

    #233867

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant

    Depositor

    Dieter Söll, Natalie Krahn

    Inserts

    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG) and …

    Promoter

    EM7, araBAD, and native As SelD

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-cytoFLARE1.0-TEV

    Plasmid

    #234517

    Purpose

    Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    EGFP-P2A-CaM-V5-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLX208-cytoFLARE1.0-TF

    Plasmid

    #234513

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLAS2w.Phyg-HAVCR1

    Plasmid

    #232669

    Purpose

    expresses HAVCR1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Insert

    HAVCR1 (HAVCR1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-pCAG eGFP T2A SP HALO HAPLN1

    Plasmid

    #228200

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    constitutive CAG promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. MZB117

    Plasmid

    #228481

    Purpose

    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    GST (glutathione S-transferase)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDM069

    Plasmid

    #216821

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold2
    

    Use

    Tags

    RIPGLIN and VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK7187

    Plasmid

    #219761

    Purpose

    MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid

    #216695

    Purpose

    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. DRD1-DuET

    Plasmid

    #213227

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD1 (DRD1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. PB-3xCTCF-TetO

    Plasmid

    #213023

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    3xCTCF-TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. p663-UBC-miniTurbo-V5-RIOK1_IDG-K

    Plasmid

    #214464

    Purpose

    Gateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-RIOK1 (RIOK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. CL20_mEGFP_LEF1_TAF8

    Plasmid

    #205846

    Purpose

    Express mEGFP-tagged fusion protein, LEF1_TAF8 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    LEF1_TAF8 (LEF1, TAF8 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.1 LARP7-FLAG 7SK

    Plasmid

    #200733

    Purpose

    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK

    Depositor

    Claudio Alarcón

    Insert

    LARP7 and 7SK (LARP7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-lox-roxSTOProx-tRFP-pA-lox

    Plasmid

    #196888

    Purpose

    Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    turbo (t)RFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET41c-PhTET2

    Plasmid

    #182428

    Purpose

    Expresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coli

    Depositor

    Bruno Franzetti

    Article

    Gauto et al Nat Commun. 2022 Apr 8;13(1):1927. doi:

    Insert

    Tetrahedral aminopeptidase TET2 from P. hirokoshii
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. DCAF1:PBC020-D05:C246337

    Plasmid

    #183974

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    DCAF1:PBC020-D05:C246337 (DCAF1 Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV and MHHHHHHSSGRENLYFQG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. AiP1181-Ai224 (TICL-nls-EGFP-ICF-nls-dTomato)

    Plasmid

    #181760

    Purpose

    targeting vector

    Depositor

    Tanya Daigle

    Insert

    nls-tagged EGFP and nls-tagged dTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2-XTbx5-EnR

    Plasmid

    #117249

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1999 Apr;126(8):1739-51.

    Insert

    Tbx5-EnR (tbx5-b Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    chimeric protein with engrailed repressor domain…

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBS mNotch1-IC (DM#270)

    Plasmid

    #15079

    Depositor

    Douglas Melton

    Article

    Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

    Insert

    Notch1-IC (Notch1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K1750A

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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