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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 65,062 results for: lat

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Showing: 7881 - 7900 of 65062 results

  3. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid
    #140984
    Purpose
    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha15-RLuc8 (GNA15 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140984-plasmid-map-sequence-id-300775

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  4. pTP434

    Plasmid
    #104167
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling
    Depositor
    Dirk Görlich
    Article
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    3x Cysteines located at bps 493-495, 520-522, and…
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104167-plasmid-map-sequence-id-200061

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  7. pCAG_EGFP-GFE3

    Plasmid
    #79869
    Purpose
    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.
    Depositor
    Don Arnold
    Article
    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm
    Insert
    GFP-GFE3
    Use
    Synthetic Biology
    Tags
    GFP and HA
    Expression
    Mammalian
    Promoter
    Beta-actin
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79869-plasmid-map-sequence-id-153506

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  11. pEGIP*35

    Plasmid
    #26776
    Purpose
    Enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting
    Depositor
    Linzhao Cheng
    Article
    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
    Insert
    GFP*35
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 29, 2010
    Availability
    Academic Institutions and Nonprofits only
    26776-plasmid-map-sequence-id-12674

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  18. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

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  20. AICSDP-25:MAP1LC3B-mEGFP

    Plasmid
    #101783
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B
    Depositor
    Allen Institute for Cell Science
    Insert
    MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101783-plasmid-map-sequence-id-197671

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Showing: 7881 - 7900 of 65062 results