We narrowed to 22,843 results for: TES

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BMR1_02g00320-bio-His

Plasmid

#108028

Purpose

Expresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

Insert

BMR1_02g00320

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaCoiledCoil-LIN-41

Plasmid

#52720

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 328–447

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCRII-Plxnb2-ISH-2kb

Plasmid

#68036

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 C548A

Plasmid

#126592

Purpose

Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C548A

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP*

Plasmid

#41665

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Arl6ip6

Plasmid

#120235

Purpose

Expresses V5-tagged Arl6ip6 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Arl6ip6 (Arl6ip6 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMIR-31-Reporter

Plasmid

#71871

Purpose

Mammalian expression vector for the analysis of miR-31 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-31

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4-Cluster Promoter Delta 2

Plasmid

#100127

Purpose

It contains ~4.8 Kb of the miR-183-96-182 cluster promoter

Depositor

Eva Hernando

Article

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

miR-183-96-82 Cluster promoter 4.8Kb Fragment (MIR182 Human)

Use

Luciferase

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4-Cluster Promoter Delta 3

Plasmid

#100128

Purpose

It contains ~2.7 Kb of the miR-183-96-182 cluster promoter

Depositor

Eva Hernando

Article

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

miR-183-96-82 Cluster promoter 2.7Kb Fragment (MIR182 Human)

Use

Luciferase

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b Me31B-3Myc

Plasmid

#113001

Purpose

For protein expression and purification of Myc-tagged full-length Drosophila me31B

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length me31B (me31B Fly)

Tags

Myc

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 P12L

Plasmid

#11020

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 P12L (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

P12L

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 A309P

Plasmid

#11023

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 A309P (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A309P

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150-MIRΔ

Plasmid

#160995

Purpose

Express mouse p150 protein with MIR deletion mutation

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

MIR (HP1-interacting domain) deletion

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150-PIP2Δ

Plasmid

#160994

Purpose

Express mouse p150 protein with PIP2 deletion mutation

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

PIP2 motif deletion

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAdTrack shALAS-1

Plasmid

#22748

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shALAS-1 (Alas1 Mouse)

Use

Adenoviral and RNAi

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT H335G

Plasmid

#13749

Depositor

Luca Pellegrini

Article

Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic His 335 to Gly

Available Since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
Brm BDmut-Flag pShuttle

Plasmid

#122108

Purpose

Expression of full length Brm containing point mutations in its bromodomain in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brm (SMARCA2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

Plasmid

#138368

Purpose

Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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2xHA MED10 pBacPAK8

Plasmid

#17321

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

MED10 (Med10 Mouse)

Tags

2xHA

Expression

Insect

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-vsv-Incenp wt-GFP

Plasmid

#108502

Purpose

inducible expression of vsv-INCENP wt-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-RNF8-N-terminus(1-111)

Plasmid

#64674

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Only N terminus amino acids 1-111

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA6-N-HA-Ubac2

Plasmid

#123598

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

ubiquitin associated domain containing 2 (Ubac2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER

Plasmid

#16753

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3-deltaCTER (DVL3 Human)

Tags

flag

Expression

Mammalian

Mutation

delta CTER

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 WT

Plasmid

#126591

Purpose

Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant to GAGGAGAUAUUCAGACAUU

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a-CRY2PHR-SID4X

Plasmid

#63663

Purpose

AAV expression of CRYS2PHR-SID4X

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Insert

SID4X-CYR2PHR

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-TBX1wt-IDR

Plasmid

#145254

Purpose

Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBX1wt-IDR (TBX1 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST-p70b-UTR1-deGFP-6xHis-T500

Plasmid

#92222

Purpose

Expression of deGFP using the p70b promoter (weak version of the p70a promoter)

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70b (p70a promoter with one mutation)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_m-AKAR5’

Plasmid

#114140

Purpose

Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5' C-terminally tagged by KRas C tail

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tac-delta scr

Plasmid

#80019

Purpose

Mammalian expression of Tac-delta scr

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tac (IL2RA Human)

Tags

KLRFGFK

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLRFGFK

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp

Plasmid

#108482

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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