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pH2B-Electra2

Plasmid

#179479

Purpose

Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

Plasmid

#91719

Purpose

AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

Depositor

Ryohei Yasuda

Publication

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2(R5AR6A)

Use

AAV

Promoter

CaMKII 1.3

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PAsfCherry2(1-10)

Plasmid

#82612

Purpose

Express PAsfCherry2(1-10) in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PAsfCherry2(1-10)

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

CMV

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-FADD

Plasmid

#78802

Purpose

To overexpress FADD in Mammalian Cells

Depositor

Jaewhan Song

Publication

Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

Insert

FADD (FADD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-flex-oChIEF-citrine

Plasmid

#50973

Purpose

flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

Depositor

Roger Tsien

Publication

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

inverted and inserted between two lox sites

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMSCV-BTK-mCherry

Plasmid

#50043

Purpose

Expression of BTK-mCherry in mammalian cells

Depositor

Hidde Ploegh

Publication

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

BTK (Btk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Publication

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Tags

Flag

Expression

Mammalian

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBSFI-myr-akt-T308D/S473D

Plasmid

#49192

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

Depositor

Peter Vogt

Publication

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308D; S473D

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pACAGW-oChiEFtdTomato-AAV

Plasmid

#32846

Purpose

AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

Depositor

Rory McQuiston

Publication

Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

Insert

oChiEF

Use

AAV

Tags

tdTomato

Promoter

CAGG

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-6P-SEW-YC3.6

Plasmid

#31379

Depositor

Fritjof Helmchen

Publication

Minderer et al J Physiol. 2011 Nov 14.

Insert

Yellow Cameleon 3.60

Use

AAV

Tags

Venus and eCFP

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FRB-myc

Plasmid

#20228

Depositor

Carolyn Bertozzi

Publication

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

T2098L point mutation

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pMKO.1 GFP B55 alpha shRNA 3-1

Plasmid

#10679

Depositor

William Hahn

Publication

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha shRNA (PPP2R2A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBABE MDER

Plasmid

#13494

Depositor

Stephen Tapscott

Publication

Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

full length MyoD was fused to aa282-595 of Esr1. …

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GZnP3 in pcDNA3.1+

Plasmid

#161738

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Publication

Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GZnP3

Expression

Mammalian

Promoter

CMV

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLENTI_RPS3_Keima

Plasmid

#127140

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLentiCRISPRv2 Neo

Plasmid

#127644

Purpose

Empty pLentiCRISPRv2 with NeoR

Depositor

Nicolas Manel

Publication

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Type

Empty backbone

Use

Lentiviral

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMXsIP GFP-VMP1

Plasmid

#117417

Purpose

Expresses VMP1 tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Publication

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

VMP1 (VMP1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-SpCas9-HF1

Plasmid

#108293

Purpose

pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHairyRed

Plasmid

#69709

Purpose

Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots

Depositor

Brett Ferguson, Peter Gresshoff

Publication

Lin et al Mol Plant. 2011 May;4(3):537-45. doi: 10

Type

Empty backbone

Expression

Plant

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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Human NANOS3-P2A-mCherry knock-in targeting construct

Plasmid

#59721

Purpose

Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

Depositor

Jacob Hanna

Publication

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

P2A mCherry

Expression

Mammalian

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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