Addgene

We narrowed to 23,813 results for: SPR

Showing: 61 - 80 of 23813 results

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pLentiCRISPR_Puro_sgAAVS1

Plasmid

#187818

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSprayer

Plasmid

#165148

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

BASU-GAL4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_zeo_sgTP53

Plasmid

#183180

Purpose

pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Tags

Expression

Mutation

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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p15a_Kan_MLS_Rosa26_Short_CRISPRDel

Plasmid

#97008

Purpose

Rosa26 locus targeting vector

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pACRISPR-TP

Plasmid

#203589

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Article

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_sgCtrl#2

Plasmid

#174149

Purpose

Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_zeo_sgNT135

Plasmid

#183181

Purpose

pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_SDHB

Plasmid

#177981

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHB (SDHB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_PROX1_1+3

Plasmid

#121176

Purpose

Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

Depositor

Timo Otonkoski

Insert

Cas9-P2A-puro and U6 promoters driven expression of gRNAs

Use

CRISPR and Lentiviral

Tags

P2A-puro

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_SDHA

Plasmid

#177980

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHA (SDHA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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SpRY

Plasmid

#198544

Purpose

Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLSPR24

Plasmid

#217309

Purpose

Promoterless (SwaI Cutsite) mRuby3:amdS fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Use

Tags

mRuby3

Expression

Yeast

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLSPR25

Plasmid

#217310

Purpose

Promoterless (SwaI Cutsite) mNeonGreen:amdS fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Use

Tags

mNeonGreen

Expression

Yeast

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLSPR30

Plasmid

#217315

Purpose

GAL7(p)-mRuby3:amdS fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Use

Tags

mRuby3

Expression

Yeast

Mutation

Promoter

GAL7

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLSPR31

Plasmid

#217316

Purpose

TEF1(p)-mRuby3:amdS fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Use

Tags

mRuby3

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLSPR32

Plasmid

#217317

Purpose

GAL7(p)-mNeonGreen:amdS fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Use

Tags

mNeonGreen

Expression

Yeast

Mutation

Promoter

GAL7

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLSPR33

Plasmid

#217318

Purpose

TEF1(p)-mNeonGreen:amdS fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Use

Tags

mNeonGreen

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_UQCRC2

Plasmid

#177982

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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