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Showing: 1561 - 1600 of 38736 results

  11. pCVD438

    Plasmid
    #63759
    Purpose
    eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pEV_S

    Plasmid
    #100266
    Purpose
    pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli
    Depositor
    Timothy Lu
    Article
    Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17.
    Inserts
    entD (entD Escherichia coli)
    entC
    entE
    entB
    entA
    vibH
    vibF
    cos
    Use
    Synthetic Biology; Expression, pbr322
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100266-plasmid-map-sequence-id-193235

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  23. pBS7

    Plasmid
    #83774
    Purpose
    Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    Venus
    Use
    Tags
    Venus
    Expression
    Yeast
    Mutation
    F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …
    Promoter
    Available since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    83774-plasmid-map-sequence-id-159984

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  28. pPNT6-eGFP

    Plasmid
    #71303
    Purpose
    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression
    Depositor
    Marco Musso, Gian Luca Forni
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    71303-plasmid-map-sequence-id-130367

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  34. pDH6

    Plasmid
    #83777
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y mutations added.
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83777-plasmid-map-sequence-id-160009

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  35. pfMDH_H303A

    Plasmid
    #67730
    Purpose
    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine
    Promoter
    T7
    Available since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. pfMDH_D230A

    Plasmid
    #67725
    Purpose
    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed aspartate 230 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. pfMDH_N191A

    Plasmid
    #67724
    Purpose
    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 191 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  38. pfMDH_K295A

    Plasmid
    #67726
    Purpose
    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 295 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1561 - 1600 of 38736 results