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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,424 results for: dcas9

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Showing: 781 - 800 of 2424 results

  7. pAAV-dSa-VPR

    Plasmid
    #99651
    Purpose
    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VP64-p65-RTA activator
    Mutation
    dead Cas9
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    99651-plasmid-map-sequence-id-191797

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  8. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Publication
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    73220-plasmid-map-sequence-id-134711

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  9. CYLBL_Neo_TREdCas9-KRAB

    Plasmid
    #251231
    Purpose
    TRE-dCas9-KRAB knock-in using TALEN at CLYBL locus (derived from Plasmid #220439)
    Depositor
    Gary Hon
    Insert
    TRE-dCas9 KRAB
    Use
    CRISPR and TALEN
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    D10A and H840A
    Promoter
    TRE
    Available since
    March 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    251231-plasmid-map-sequence-id-502941

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  10. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid
    #170378
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Publication
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    human Synapsin promoter
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170378-plasmid-map-sequence-id-333532

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  11. Biomolecular FeedBack (FB)

    Plasmid
    #109391
    Purpose
    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
    Depositor
    Tom Ellis
    Publication
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pJ23100mut-dCas9-T- phtpG1-sgRNA
    Expression
    Bacterial
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109391-plasmid-map-sequence-id-210784

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  12. lenti-EF1a-dCas9-VPR-Puro

    Plasmid
    #242162
    Purpose
    Lentiviral expression of dCas9-VPR with puromycin reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64-RelA-Rta; T2A-puro
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242162-plasmid-map-sequence-id-496080

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  17. pJK413

    Plasmid
    #155372
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    155372-plasmid-map-sequence-id-309000

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  20. lenti-EF1a-dCas9-KRAB-MeCP2-H2B-mCherry

    Plasmid
    #242171
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with H2B-mCherry reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-H2B-mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Feb. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    242171-plasmid-map-sequence-id-499156

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Showing: 781 - 800 of 2424 results