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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,441 results for: dcas9

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Showing: 781 - 800 of 2441 results

  3. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Publication
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

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  11. pMT423

    Plasmid
    #139382
    Purpose
    Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR
    Depositor
    Christopher Voigt
    Publication
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Constitutively expressed dCas9, LacI and TetR
    Use
    Synthetic Biology
    Promoter
    P1
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    139382-plasmid-map-sequence-id-313187

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  13. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid
    #170378
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Publication
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    human Synapsin promoter
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170378-plasmid-map-sequence-id-333532

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  14. pAAV-dSa-VPR

    Plasmid
    #99651
    Purpose
    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VP64-p65-RTA activator
    Mutation
    dead Cas9
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    99651-plasmid-map-sequence-id-191797

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  15. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Publication
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73220-plasmid-map-sequence-id-134711

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  16. CYLBL_Neo_TREdCas9-KRAB

    Plasmid
    #251231
    Purpose
    TRE-dCas9-KRAB knock-in using TALEN at CLYBL locus (derived from Plasmid #220439)
    Depositor
    Gary Hon
    Insert
    TRE-dCas9 KRAB
    Use
    CRISPR and TALEN
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    D10A and H840A
    Promoter
    TRE
    Available since
    March 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    251231-plasmid-map-sequence-id-502941

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  18. Biomolecular FeedBack (FB)

    Plasmid
    #109391
    Purpose
    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
    Depositor
    Tom Ellis
    Publication
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pJ23100mut-dCas9-T- phtpG1-sgRNA
    Expression
    Bacterial
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109391-plasmid-map-sequence-id-210784

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Showing: 781 - 800 of 2441 results