167,642 results

1. pICE-HA-NLS-I-PpoI

   Plasmid

   #46963

   Purpose

   For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   NLS-I-PpoI
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV-TET

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPUR-mU6-sgRNA-2XPP7

   Plasmid

   #194504

   Purpose

   Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

   Depositor

   Chunqing Song

   Article

   Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-HA-CDK4

   Plasmid

   #172612

   Purpose

   Expresses HA-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mRuby2

   Plasmid

   #40260

   Purpose

   RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Insert

   mRuby2
   

   Tags

   His6 and Xpress

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

   Plasmid

   #216156

   Purpose

   Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with C-terminal FLAG tag
   

   Expression

   Mammalian

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

   Plasmid

   #223174

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   RfxCas13d
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZac2.1 gfaABC1D-eGFP

   Plasmid

   #176861

   Purpose

   Expresses cytosolic eGFP in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Bacterial

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB533-44B12m23-sfGFP

   Plasmid

   #186778

   Purpose

   RNAP2 Ser5ph-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   44B12m23-scFv
   

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038)

    Plasmid

    #191720

    Purpose

    AI plasmid ALIAS: AiP13038. Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Mutation

    none

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101297

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-906 of alpha-Catenin

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_BiSSTe10_C1V1_eYFP

    Plasmid

    #213816

    Purpose

    AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_C1V1_eYFP

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    C1V1-eYFP
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. myc-Rictor corrected

    Plasmid

    #11367

    Purpose

    Mammalian expression of myc tagged human RICTOR

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Rictor (RICTOR Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    April 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGKp-GFP-YAP

    Plasmid

    #174172

    Purpose

    Lentiviral vector to constitutively express YAP under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMXS-mitoGshF-blasticidin

    Plasmid

    #210314

    Purpose

    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

    Depositor

    Kivanc Birsoy

    Article

    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

    Insert

    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    

    Use

    Retroviral

    Tags

    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

    Mutation

    Codon optimized for expression in human cells

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_RTN3E-mScarlet3

    Plasmid

    #216255

    Purpose

    mammalian expression vector for RTN3E-mScarlet3

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    reticulon 3 isoform e (RTN3 Human)
    

    Tags

    mScarlet3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3_cAMPFIRE-M

    Plasmid

    #182280

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEE082 FoMV

    Plasmid

    #234811

    Purpose

    To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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