We narrowed to 101,739 results for: %s

1. pGL3-U6-pegRNA_SRD5A3-EGFP

   Plasmid

   #159781

   Purpose

   S. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editing

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of pegRNA targeting SRD5A3 gene (SRD5A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HP21-TPI-WT-no_introns

   Plasmid

   #131044

   Purpose

   Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

   Depositor

   Marvin Tanenbaum

   Article

   Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

   Insert

   TPI-Wildtype-no introns (TPI1 Human)
   

   Expression

   Mammalian

   Promoter

   Pcmv

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFX-Lifeact-mCherry

   Plasmid

   #175822

   Purpose

   Express mCherry with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with actin-targeting sequence
   

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Promoter

   CMB

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-U6-sgRNA_SRD5A3-mcherry

   Plasmid

   #159782

   Purpose

   S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A3 gene

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of sgRNA targeting SRD5A3 gene (SRD5A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HP21-TPI-PTC1

   Plasmid

   #131043

   Purpose

   Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR

   Depositor

   Marvin Tanenbaum

   Article

   Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

   Insert

   TPI-PTC1 (TPI1 Human)
   

   Expression

   Mammalian

   Mutation

   PTC1

   Promoter

   Pcmv

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFX-Lifeact-iRFP

   Plasmid

   #175823

   Purpose

   Express iRFP with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with actin-targeting sequence
   

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFX-Lifeact-Venus

   Plasmid

   #174478

   Purpose

   Express Venus with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with actin-targeting sequence
   

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-U6-sgRNA_EMX1-mcherry

   Plasmid

   #159784

   Purpose

   S. pyogenes sgRNA collocated with pegRNA targeting human EMX1 gene

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of sgRNA targeting EMX1gene (EMX1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFX-Lifeact-EGFP

   Plasmid

   #174477

   Purpose

   Express EGFP with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with actin-targeting sequence
   

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFX-Lifeact-SECFP

    Plasmid

    #174476

    Purpose

    Express SECFP with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with actin-targeting sequence
    

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFX-Lifeact-Sirius

    Plasmid

    #174475

    Purpose

    Express Sirius with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with actin-targeting sequence
    

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330S-2-PITCh

    Plasmid

    #63670

    Purpose

    Expresses Cas9 nuclease and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRPN265NB-LaG16-MNase

    Plasmid

    #170978

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

    Insert

    Glutathione-S-Transferase/LaG16/MNase
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24b-Amg2

    Plasmid

    #199434

    Purpose

    expresses Amg2 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    Amg2
    

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    L159K mutation

    Promoter

    T7

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330A_D10A-1x5

    Plasmid

    #58775

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BHMT

    Plasmid

    #64075

    Purpose

    Expression of betaine homocysteine methyltransferase

    Depositor

    Vern Schramm

    Article

    Poulin et al J Org Chem. 2015 May 15;80(10):5344-7. d

    Insert

    betaine-homocysteine methyltransferase (BHMT Human, Synthetic)
    

    Tags

    none

    Expression

    Bacterial

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330A_D10A-1x7

    Plasmid

    #58777

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330A_D10A-1x4

    Plasmid

    #58774

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 4A3.SU

    Plasmid

    #197675

    Purpose

    Expresses P1 S tail fibre and U chaperone

    Depositor

    Baojun Wang

    Article

    Huan et al J Mol Biol. 2022 Sep 15;434(21):167829.

    Inserts

    S
    U
    

    Expression

    Bacterial

    Promoter

    Late S promoter, LPS and N/A

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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