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We narrowed to 24,928 results for: CRISPR-Cas9

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Showing: 8101 - 8120 of 24928 results

  6. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64710-plasmid-map-sequence-id-108923

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  7. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166700-plasmid-map-sequence-id-329224

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  8. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  9. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

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  10. Adeno EA

    Plasmid
    #64071
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Insert
    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Promoter
    U6 and CBh
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pKMW281_dSpRY

    Plasmid
    #244822
    Purpose
    Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)
    Depositor
    Jennifer Doudna
    Article
    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.
    Insert
    dSpRY Cas9
    Use
    CRISPR
    Tags
    3xFLAG-SV40 NLS and Nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CAG
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244822-plasmid-map-sequence-id-486583

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  13. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  15. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

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  16. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    85401-plasmid-map-sequence-id-322305

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  17. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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  20. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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Showing: 8101 - 8120 of 24928 results