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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,777 results for: SEC

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Showing: 8161 - 8180 of 10777 results

  5. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62857-plasmid-map-sequence-id-212900

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  6. pCB’

    Plasmid
    #162703
    Purpose
    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Expression
    Bacterial
    Mutation
    5513T>G (tetR E37A); 7758G>T (second tet op…
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162703-plasmid-map-sequence-id-324199

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  8. pFastBacDual with LFn + Needle

    Plasmid
    #84868
    Purpose
    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Needle
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84868-plasmid-map-sequence-id-162901

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  9. pSL0284 (pQCascade_entry)

    Plasmid
    #130635
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130635-plasmid-map-sequence-id-255022

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  11. AAEL010097-Cas9

    Plasmid
    #100707
    Purpose
    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Promoter
    AAEL010097 and Opie2
    Available Since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100707-plasmid-map-sequence-id-193540

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  15. PCYOX1L-bio-His

    Plasmid
    #53410
    Purpose
    Expresses full-length Prenylcysteine oxidase-like precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PCYOX1L (PCYOX1L Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53410-plasmid-map-sequence-id-83193

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  19. pC5Kan-P2A

    Plasmid
    #51814
    Purpose
    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message
    Depositor
    Barry Ganetzky
    Article
    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:
    Insert
    P2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
    Use
    Shuttle vector for insect expression vectors
    Mutation
    codons altered for Drosophila and to eliminate re…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    51814-plasmid-map-sequence-id-79554

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  20. pCCM’

    Plasmid
    #162709
    Purpose
    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Mutation
    p15A origin replaced with high-copy colE1 origin
    Promoter
    PLtet0-1 promoter
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162709-plasmid-map-sequence-id-341131

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Showing: 8161 - 8180 of 10777 results