We narrowed to 11,368 results for: nls

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJM573 ZF6x6-C 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161560

Purpose

Inducible expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the ZF6x6-C promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

ZF6x6-C

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM576 ZF2x6-C 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161562

Purpose

Inducible expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the ZF2x6-C promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

ZF2x6-C

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM524 EF1α 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161553

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM525 EF1α 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161554

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-FNLS-PGK-Puro

Plasmid

#110847

Purpose

Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

TRE3G

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hGFAP-NLS-HA-Dre-P2A-BFP

Plasmid

#51277

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

Depositor

Pawel Pelczar

Publication

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Dre-P2A-BFP

Expression

Mammalian

Promoter

hGFAP

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

Plasmid

#121966

Purpose

Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

scFv-GCN4

Use

Lentiviral

Tags

superfold GFP-GB1-NLS-iLID

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xPCP-2xGFP

Plasmid

#71243

Purpose

Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

Depositor

Anne Ephrussi

Publication

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

2xPCP-2xGFP

Use

P element-mediated germline transformation

Tags

EGFP

Expression

Insect

Promoter

Hsp83

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YIPlac204TC-NLS-DsRed-Express2

Plasmid

#25443

Depositor

Benjamin Glick

Publication

Strack et al Nat Methods. 2008 Nov . 5(11):955-7.

Insert

NLS-DsRed-Express2

Tags

NLS

Expression

Yeast

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

Plasmid

#121966

Purpose

Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

scFv-GCN4

Use

Lentiviral

Tags

superfold GFP-GB1-NLS-iLID

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM573 ZF6x6-C 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161560

Purpose

Inducible expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the ZF6x6-C promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

ZF6x6-C

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM576 ZF2x6-C 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161562

Purpose

Inducible expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the ZF2x6-C promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

ZF2x6-C

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM524 EF1α 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161553

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM525 EF1α 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161554

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHsp83-NLS-HA-2xPCP-2xGFP

Plasmid

#71243

Purpose

Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

Depositor

Anne Ephrussi

Publication

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

2xPCP-2xGFP

Use

P element-mediated germline transformation

Tags

EGFP

Expression

Insect

Promoter

Hsp83

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge