We narrowed to 6,239 results for: nls

1. PS Intein NLS-NES

   Plasmid

   #242021

   Purpose

   Blue-light-induced reconstitution of mCherry and its relocalization to the nucleus.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

   Insert

   PS Intein NLS-NES
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS

   Plasmid

   #244843

   Purpose

   Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Lbm6-Cas12a
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 4xSV40 NLS

   Expression

   Bacterial

   Mutation

   D535G/S551F/D665N

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3TS-RfxCas13d-2C-NLS

   Plasmid

   #239816

   Purpose

   Plasmid to carry out IVT of RfxCas13d with SV40 and nucleoplasmin long-NLP NLS (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   Nucleoplasmin long NLS and SV40 NLS

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTcTurboID-GW_GFP-NLS

   Plasmid

   #234716

   Purpose

   Used to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling nuclear spatial control.

   Depositor

   Esteban Serra

   Insert

   GFP-3xNLS
   

   Use

   Trypanosomatid expression

   Tags

   3xHA

   Promoter

   T7

   Available Since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-TDP-43 (PY-NLS)

   Plasmid

   #235490

   Purpose

   Expression of chimeric human TDP-43-EGFP bearing a PY-nuclear localization sequence (NLS) from HNRNPA1 in place of the native NLS sequence

   Depositor

   Maxime Rousseaux

   Article

   Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

   Insert

   TARDBP (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Replaced native NLS with PY-NLS from HNRNPA

   Available Since

   May 19, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. UAST-uMCas12a+nls2x

   Plasmid

   #230909

   Purpose

   inducible expression of Cas12a+ with Gal4.

   Depositor

   Michael Boutros

   Article

   Port et al bioRxiv 2024.11.26.625385

   Insert

   Cas12a+
   

   Use

   CRISPR

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV_dCas9-AsLOV2x3-NLSx3

   Plasmid

   #231567

   Purpose

   Expresses components of the LOOMINA optogenetic transcriptional control system.

   Depositor

   Dominik Niopek

   Article

   Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

   Insert

   dCas9-AsLOV2x3
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC3-C-GFP-NLS

   Plasmid

   #222604

   Purpose

   Expression of MAC3-tagged GFP-NLS (C-terminal)

   Depositor

   Markku Varjosalo

   Article

   Liu et al Methods Mol Biol. 2023;2690:281-297. doi

   Insert

   EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
   

   Tags

   MAC3

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Aug. 6, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. MAC3-N-GFP-NLS

   Plasmid

   #222607

   Purpose

   Expression of MAC3-tagged GFP-NLS (N-terminal)

   Depositor

   Markku Varjosalo

   Article

   Liu et al Methods Mol Biol. 2023;2690:281-297. doi

   Insert

   EGFP and 3' nuclear localizaition sequence (NLS)
   

   Tags

   MAC3

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLenti-LbCas12a-8xNLS

    Plasmid

    #209020

    Purpose

    Lentiviral vector expressing human codon-optimized LbCas12a with 8xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    LbCas12a-8xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-NLS-HA-TurboID

    Plasmid

    #215073

    Purpose

    Expresses NLS-HA-TurboID in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. NLS-mAzGreen-Cyclin D1

    Plasmid

    #215071

    Purpose

    Expresses NLS-mAzGreen-Cyclin D1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    NLS-mAzGreen

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. NLS-mAzGreen-Cyclin D1 (HP)

    Plasmid

    #215072

    Purpose

    Expresses NLS-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    NLS-mAzGreen

    Expression

    Mammalian

    Mutation

    Cyclin D1(HP) denotes mutations M56A, V60A, and W…

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. L_NLS 41kDa (BFP)

    Plasmid

    #201392

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Insert

    (NLS)SV40A4-BFP-2PrA
    

    Expression

    Mammalian

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLipoP1-NLS-3xmCitrine

    Plasmid

    #192522

    Purpose

    Fluorescent reporter for plant responses to bacterial infection

    Depositor

    Gitta Coaker

    Article

    Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

    Insert

    Putative membrane lipoprotein (AT3G18250 Mustard Weed)
    

    Tags

    3xmCitrine and NLS

    Expression

    Plant

    Promoter

    LipoP1

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEXPA10-NLS-3xmCitrine

    Plasmid

    #192523

    Purpose

    Fluorescent reporter for plant responses to bacterial infection

    Depositor

    Gitta Coaker

    Article

    Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

    Insert

    EXPANSIN A10 (EXPA10 Mustard Weed)
    

    Tags

    3xmCitrine and NLS

    Expression

    Plant

    Promoter

    EXPA10

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPIP1;4-NLS-3xmCitrine

    Plasmid

    #192524

    Purpose

    Fluorescent reporter for plant responses to bacterial infection

    Depositor

    Gitta Coaker

    Article

    Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

    Insert

    PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)
    

    Tags

    3xmCitrine and NLS

    Expression

    Plant

    Promoter

    PIP1;4

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LFNG:GFPnls-T2a-ER-Cre-ER

    Plasmid

    #200107

    Depositor

    Prayag Murawala

    Insert

    LFNG:GFPnls-T2A-ER-Cre-ER
    

    Use

    Unspecified

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMpGWBhis03-Citrine-NLS

    Plasmid

    #188558

    Purpose

    Binary vector for the expression of Citrine-NLS with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

    Depositor

    Yutaka Kodama

    Article

    Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

    Insert

    Citrine-NLS and MpIGPDm
    

    Use

    CRISPR; Entry vector

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLS-Halo-HSF2

    Plasmid

    #200198

    Purpose

    Expression of full length HSF2 with SV40 NLS

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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