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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,221 results for: gnas

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Showing: 8181 - 8200 of 10221 results

  5. pCX-SpiCee-Kras

    Plasmid
    #140838
    Purpose
    mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Article
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Insert
    SpiCee-Kras
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140838-plasmid-map-sequence-id-304816

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  9. ULK1-Halo HRD

    Plasmid
    #207548
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    207548-plasmid-map-sequence-id-430489

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  10. pcDNA3-nuc-Nb80

    Plasmid
    #205519
    Purpose
    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb80
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205519-plasmid-map-sequence-id-409422

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  15. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid
    #224583
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224583-plasmid-map-sequence-id-451495

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  19. pYFP-NEAT1m_v1-RT

    Plasmid
    #97089
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1m_v1-RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97089-plasmid-map-sequence-id-189689

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  20. pYFP-NEAT1_IS_RT

    Plasmid
    #97091
    Purpose
    Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1_IS_RT (NEAT1 Human)
    Use
    CRISPR
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97091-plasmid-map-sequence-id-189691

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Showing: 8181 - 8200 of 10221 results