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pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

Plasmid

#85453

Purpose

Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

Depositor

Aviv Regev

Article

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-sgRNA-Backbone-UP-TdTomato

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

deletion of AA123-365 in tdTomato (please see dep…

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGATOR

Plasmid

#64102

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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untargeted Plk1 FRET sensor Myt1 substrate

Plasmid

#45217

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Plk1 FRET sensor Myt1 substrate

Expression

Mammalian

Promoter

t7

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pME-puro-Venus-FLAG-CD59

Plasmid

#50377

Purpose

Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Article

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

Venus

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pRVdG-EGFP-N-P-M-SynPhRFP-L

Plasmid

#52479

Purpose

Expresses EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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p6661 MSCV-IP N-HA only 16E6

Plasmid

#42603

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pSicoR-Ef1a-mCh-GFPi

Plasmid

#31849

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223-PBK

Plasmid

#23782

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PBK (PBK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDmelOR-mRho.V5.mER.hOr56a

Plasmid

#126479

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or56a (Or56a Fly)
Orco (Orco Fly)

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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TurboID-CRaf

Plasmid

#226168

Purpose

Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-TurboID-CRaf (RAF1 Human)

Use

Lentiviral

Tags

EGFP-IRES-BlastR and Myc-TurboID

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-2-GFP

Plasmid

#204202

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

multiple silent mutations

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mPD-L1-T2A-GFP

Plasmid

#205455

Purpose

lentiviral plasmid for expression of mouse PD-L1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mPD-L1 (Cd274 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TEK-V5/HIS

Plasmid

#205611

Purpose

Expression of human TEK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TEK receptor tyrosine kinase (TEK Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR Grb2-TagBFP

Plasmid

#188631

Purpose

Human Grb2 C-terminally labeled with TagBFP

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

GRB2 (GRB2 Human, Synthetic)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pUb PspCas13b + PspCas13b guide RNA

Plasmid

#176305

Purpose

Expresses PspCas13b and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

PspCas13b

Tags

HA tag

Expression

Insect

Mutation

E113K, N265S- please depositor comments

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#165491

Purpose

Neuronal expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity .

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc

Plasmid

#158603

Purpose

Plasmid expressing KB-1753-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTet-O-E2F7-T2A-PuroR

Plasmid

#162348

Purpose

Lentiviral expression of E2F7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

E2F7 (E2F7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

Plasmid

#162333

Purpose

Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSpCas9-2xVP64

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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