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Plasmid#198647PurposeEasy-MISE toolkit pEM-plasmid containing TPI1 promoter with EF protruding endsDepositorInsertpTPI1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P03R
Plasmid#198646PurposeEasy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding endsDepositorInsertpPGK1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P03L
Plasmid#198645PurposeEasy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding endsDepositorInsertpPGK1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P02R
Plasmid#198644PurposeEasy-MISE toolkit pEM-plasmid containing ENO2 promoter with FG protruding endsDepositorInsertpENO2
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P02L
Plasmid#198643PurposeEasy-MISE toolkit pEM-plasmid containing ENO2 promoter with EF protruding endsDepositorInsertpENO2
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P01R
Plasmid#198642PurposeEasy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding endsDepositorInsertpTDH3
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P01L
Plasmid#198641PurposeEasy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding endsDepositorInsertpTDH3
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT2_32_PyruEth_X2site
Plasmid#194428PurposePlasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)DepositorInsertPyruEth
UseUsed as donor dna in genome integration after linā¦TagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT1_75_Donor_Tag_RPL13A-mTurq2
Plasmid#194415PurposePlasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRproDepositorInsertmTurquoise2 CDS
UseSynthetic Biology; Used as donor dna in genome inā¦TagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPINA7A2mTFP1(F)
Plasmid#193774PurposeExpresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertmTFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPINA7 synthetic promoter carrying binding sites fā¦Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
KBB441
Plasmid#185131PurposeSwi6 full length with L->A mutated in nuclear localization signalDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSWI6 deletion of amino acids 450-458PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB556
Plasmid#185108Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160ADepositorInsertPOM152
UseTagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domaiā¦PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB553
Plasmid#185107Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182LDepositorInsertPOM152
UseTagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domaiā¦PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9341 (pgRNA_IX-1_NatMX)
Plasmid#161593PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9343 (pgRNA_XV-1_NatMX)
Plasmid#161595PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB345
Plasmid#185078Purposerfa3D46 truncation fused to GFPDepositorInsertRFA3
UseTagsGFPExpressionYeastMutationRfa3 truncation aa 1-46PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB419
Plasmid#185089PurposeSwi6L-GFP expressing N-term 273 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation at aa 273PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB354
Plasmid#185082PurposeSwi6L-GFP expressin N-term 273 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation aa 1-273PromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB416
Plasmid#185087PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB349
Plasmid#185080Purposerfa2D248 truncation fused to GFPDepositorInsertRFA2
UseTagsGFPExpressionYeastMutationRfa2 truncation aa 1-247PromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB353
Plasmid#185081PurposeSwi6M-GFP expressing N-term 181 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation aa 1-181PromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB484
Plasmid#185132PurposeSWI6-GFP full length under control of GAL1 promotionDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB486
Plasmid#185133PurposeFull length CRM1 fused to binding domain of two-hybrid plasmidDepositorInsertCRM1
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB491
Plasmid#185134PurposeFull length SWI6 fused to activation domain of two-hybrid plasmidDepositorInsertSWI6
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS105
Plasmid#174830PurposeEncodes a unique yeast telomere end at TEL11R by removing the subtelomereDepositorInsert11R Unique
UseTagsGAL4 5XUASExpressionBacterialMutationChanging TEL11R to a unique endPromoterlac promoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-197
Plasmid#169924PurposeWT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2DepositorInsertEcm2 1-197
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJC8
Plasmid#170757PurposeCo-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.DepositorTypeEmpty backboneUseTagssite 1- V5 tag and site 2- FLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
A102-GAP-BoCCS
Plasmid#123287PurposeExpression of truncated Glo3 (1-375) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation1-375PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS-GRM
Plasmid#123288PurposeExpression of truncated Glo3 (137-459) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation137-459PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-Par-BoCCS-GRM
Plasmid#123289PurposeExpression of truncated Glo3 (296-459) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation296-459PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS
Plasmid#123290PurposeExpression of truncated Glo3 (137-375) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation137-375PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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S3_009_026_MV1
Plasmid#134421PurposepYTKMV1-pREV1_ NLS_FdeR-N_2ļ“NLS-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_009_026_096
Plasmid#134412PurposepYTK096-pREV1_ NLS_FdeR-N_2ļ“NLS-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_006_026_096
Plasmid#134409PurposepYTK096-pREV1_FdeR-N_2ļ“NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pREV1Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_006_026_096
Plasmid#134418PurposepYTK096-pREV1_FdeR-N_2ļ“NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_003_026_096
Plasmid#134406PurposepYTK096-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pREV1Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_003_026_MV1
Plasmid#134415PurposepYTKMV1-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-pol30(K127/164R)
Plasmid#99545PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationK127R, K164RPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTCW022
Plasmid#83532Purposepheromone mediated expression of UBiC, ARO4, and TKL1DepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW015
Plasmid#83530PurposeUBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoterDepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHBA03
Plasmid#83537PurposeConstitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genesDepositorInsertpTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-T-TEF2
Plasmid#65340Purposeconstruction of TEF2 terminator in HCKan-P receiving vectorDepositorInsertTEF2 terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-P-TDH3
Plasmid#65336Purposeconstruction of TDH3 promoter in HCKan-P receiving vectorDepositorInsertTDH3 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-T-ADH1
Plasmid#65339Purposeconstruction of ADH1 terminator in HCKan-P receiving vectorDepositorInsertADH1 terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD125 GFP:POP1
Plasmid#71387Purposelocalization of yeast RNase POP1 (RNase MRP binding protein) in yeastDepositorInsertPOP1 (POP1 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterACT1Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-P-CYC1
Plasmid#65338Purposeconstruction of CYC1 promoter in HCKan-P receiving vectorDepositorInsertCYC1 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS2-Pleum
Plasmid#59992PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promoteā¦TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tpa1(273-644)
Plasmid#23141DepositorInsertTpa1 C-terminal domain (TPA1 Budding Yeast)
UseTagsHisExpressionBacterialMutationdeleted amino acids 1-272PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only