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Bacterial Strain#49018PurposeThis is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 CDepositorBacterial ResistanceBleocin (zeocin)SpeciesEscherichia coliAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only
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C321.ΔA
Bacterial Strain#48998PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)DepositorBacterial ResistanceAmpicillinSpeciesEscherichia coliAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
C321.deltaA (Isaacs lab)
Bacterial Strain#73581PurposeGenomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB geneDepositorBacterial ResistanceAmpicillinAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pGLU_65
Plasmid#225768PurposeThis is the native promoter from the aac(3)-Ia gentamicin resistance cassette in IDT backboneDepositorInsertPaac(3)-Ia_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3-101-phzAG
Plasmid#165617PurposeConstitutive expression of phzAG operon for phenazine-1-carboxylic acid productionDepositorInsertphzABCDEFG
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB
Plasmid#216753PurposeOverexpression of FeoB for biological characterisationDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationWild typePromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilB
Plasmid#162845PurposeExpression of PilB with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_4ZHW
Plasmid#234158PurposeHeterologous protein expression of YfiB in Escherichia coliDepositorInsert4ZHW (yfiB Pseudomonas aeruginosa PAO1)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a(+)-PA_PhoU
Plasmid#59334PurposePho regulationDepositorInsertphoU
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBF120*
Plasmid#162852PurposeExpression of PilB F120 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationF120AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBT125*
Plasmid#162853PurposeExpression of PilB T125 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationT125AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBN189*
Plasmid#162854PurposeExpression of PilB N189 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationN189AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBL193*
Plasmid#162855PurposeExpression of PilB L193 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationL193AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBK197*
Plasmid#162856PurposeExpression of PilB K197 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationK197AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBQ231*
Plasmid#162857PurposeExpression of PilB Q231 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationQ231AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBL240*
Plasmid#162858PurposeExpression of PilB L240 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationL240AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only